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J Dermatol Sci. 2018 Sep;91(3):239-249. doi: 10.1016/j.jdermsci.2018.05.003. Epub 2018 May 21.

Proteome-wide changes in primary skin keratinocytes exposed to diesel particulate extract-A role for antioxidants in skin health.

Author information

1
Institute of Bioinformatics, International Tech Park, Bangalore 560066, India. Electronic address: pavithra@ibioinformatics.org.
2
Institute of Bioinformatics, International Tech Park, Bangalore 560066, India. Electronic address: ankit@ibioinformatics.org.
3
Institute of Bioinformatics, International Tech Park, Bangalore 560066, India. Electronic address: vishalakshi@ibioinformatics.org.
4
Institute of Bioinformatics, International Tech Park, Bangalore 560066, India. Electronic address: krishnapatel@ibioinformatics.org.
5
Institute of Bioinformatics, International Tech Park, Bangalore 560066, India. Electronic address: kiran@ibioinformatics.org.
6
Institute of Bioinformatics, International Tech Park, Bangalore 560066, India; Manipal Academy of Higher Education (MAHE), Manipal 576104, Karnataka, India. Electronic address: niraj@ibioinformatics.org.
7
L'Oréal Research and Innovation, Aulnay sous bois, 93600, France. Electronic address: ncavusoglu@rd.loreal.com.
8
L'Oréal India Pvt. Ltd., Beary's Global Research Triangle, Bangalore 560067, India. Electronic address: NITAROY@rd.loreal.com.
9
L'Oréal Research and Innovation, Aulnay sous bois, 93600, France. Electronic address: JSOEUR@rd.loreal.com.
10
L'Oréal Research and Innovation, Aulnay sous bois, 93600, France. Electronic address: LBRETON@rd.loreal.com.
11
McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA; Departments of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA; Departments of Oncology, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA; Departments of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address: pandey@jhmi.edu.
12
Institute of Bioinformatics, International Tech Park, Bangalore 560066, India. Electronic address: harsha@ibioinformatics.org.
13
Institute of Bioinformatics, International Tech Park, Bangalore 560066, India. Electronic address: aditi@ibioinformatics.org.
14
L'Oréal Research and Innovation, Aulnay sous bois, 93600, France; L'Oréal India Pvt. Ltd., Beary's Global Research Triangle, Bangalore 560067, India. Electronic address: NMISRA@rd.loreal.com.

Abstract

BACKGROUND:

Skin acts as a protective barrier against direct contact with pollutants but inhalation and systemic exposure have indirect effect on keratinocytes. Exposure to diesel exhaust has been linked to increased oxidative stress.

OBJECTIVE:

To investigate global proteomic alterations in diesel particulate extract (DPE)/its vapor exposed skin keratinocytes.

METHODS:

We employed Tandem Mass Tag (TMT)-based proteomics to study effect of DPE/DPE vapor on primary skin keratinocytes.

RESULTS:

We observed an increased expression of oxidative stress response protein NRF2, upon chronic exposure of primary keratinocytes to DPE/its vapor which includes volatile components such as polycyclic aromatic hydrocarbons (PAHs). Mass spectrometry-based quantitative proteomics led to identification 4490 proteins of which 201 and 374 proteins were significantly dysregulated (≥1.5 fold, p≤0.05) in each condition, respectively. Proteins involved in cellular processes such as cornification (cornifin A), wound healing (antileukoproteinase) and differentiation (suprabasin) were significantly downregulated in primary keratinocytes exposed to DPE/DPE vapor. These results were corroborated in 3D skin models chronically exposed to DPE/DPE vapor. Bioinformatics analyses indicate that DPE and its vapor affect distinct molecular processes in skin keratinocytes. Components of mitochondrial oxidative phosphorylation machinery were seen to be exclusively overexpressed upon chronic DPE vapor exposure. In addition, treatment with an antioxidant like vitamin E partially restores expression of proteins altered upon exposure to DPE/DPE vapor.

CONCLUSIONS:

Our study highlights distinct adverse effects of chronic exposure to DPE/DPE vapor on skin keratinocytes and the potential role of vitamin E in alleviating adverse effects of environmental pollution.

KEYWORDS:

Electron transport chain; Orbitrap Fusion; Pollution; Quantitative proteomics; Skin keratinocytes; Tocopherol

PMID:
29857962
DOI:
10.1016/j.jdermsci.2018.05.003
[Indexed for MEDLINE]
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