Abstract
Superoxide dismutase, catalase and methional proved capable of inhibiting the microsomal oxidation of thiobenzamide, which is most probably catalyzed by the flavin-containing monooxygenase. This indicates that excited oxygen species (e.g. X O-2,H2O2, X OH) are involved in the catalytic cycle of this enzymatic reaction. CO, which inhibits the cytochrome P-450-dependent oxygen radical formation, had no effect on the oxidation reaction, suggesting that the source of the reactive oxygen species is not the microsomal mixed-function oxidase.
MeSH terms
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Aldehydes / pharmacology
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Amides / metabolism*
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Animals
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Catalase / pharmacology
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Free Radicals
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Hydrogen Peroxide / metabolism
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Hydroxides / metabolism
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Hydroxyl Radical
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Male
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Microsomes, Liver / drug effects
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Microsomes, Liver / metabolism*
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Oxidation-Reduction
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Oxygen / metabolism*
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Rats
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Rats, Inbred Strains
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Superoxide Dismutase / pharmacology
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Superoxides / metabolism
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Thioamides / metabolism*
Substances
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Aldehydes
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Amides
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Free Radicals
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Hydroxides
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Thioamides
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methional
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Superoxides
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Hydroxyl Radical
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thiobenzamide
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Hydrogen Peroxide
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Catalase
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Superoxide Dismutase
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Oxygen