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Mol Nutr Food Res. 2018 May 31:e1800142. doi: 10.1002/mnfr.201800142. [Epub ahead of print]

Yerba Mate Stimulates Mitochondrial Biogenesis and Thermogenesis in High-Fat-Diet-Induced Obese Mice.

Author information

1
Unidade Integrada de Farmacologia e Gastroenterologia, Universidade São Francisco, Bragança Paulista, São Paulo, 12916-900, Brazil.
2
Programa de Pos Graduação em Genetica e Biologia Molecular, State University of Campinas,, Campinas, São Paulo, 13083-862, Brazil.
3
Nutrition and Obesity Group, Department of Nutrition and Food Science, University of the Basque Country (UPV/EHU) and Lucio Lascaray Research Institute, Vitoria, 01080, Spain.
4
CIBERobn Physiopathology of Obesity and Nutrition, Instituto de Salud Carlos III (ISCIII), 28029, Spain.
5
Neuroscience Area, Biodonostia Health Research Institute, San Sebastian, 20014, Spain.

Abstract

SCOPE:

The potential effects of yerba mate (YM) on mitochondrial biogenesis and thermogenesis are evaluated.

METHODS AND RESULTS:

The in vitro effects of YM on mitochondrial respiration are assessed in C2C12 cells. The expression of genes related to mitochondrial biogenesis and thermogenesis are analyzed by quantitative PCR. The in vivo experiments are performed on mice fed a high-fat diet (HFD) and treated with YM extract. Indirect calorimetry was performed, and the expression of genes and proteins related to mitochondrial biogenesis, thermogenesis, and de novo lipogenesis is determined by quantitative PCR and western blot. Our in vitro data indicate that YM increases mtDNA copy number as well as mitochondrial spare respiratory capacity and coupling efficiency. The gene expression profile reinforces this evidence, indicating a modulation of genes downstream of Ampk. In vivo, it is found that YM partially prevents diet-induced obesity by increasing energy expenditure and enhancing mitochondrial biogenesis via the AMPK/SIRT1/PGC1α pathway.

CONCLUSIONS:

YM stimulates mitochondriogenesis and Ucp expression, leading to an increase in the spare respiratory capacity and energy dissipation. These effects may help to better understand the potential use of YM for obesity treatment.

KEYWORDS:

Ilex paraguariensis; de novo lipogenesis; mitochondrial biogenesis; obesity; thermogenesis

PMID:
29851217
DOI:
10.1002/mnfr.201800142

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