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Methods Mol Biol. 2018;1782:287-299. doi: 10.1007/978-1-4939-7831-1_16.

Plate-Based Measurement of Superoxide and Hydrogen Peroxide Production by Isolated Mitochondria.

Author information

1
Buck Institute for Research on Aging, Novato, CA, USA.
2
Helen and Robert Appel Alzheimer's Disease Research Institute, Feil Family Brain and Mind Research Institute, Weill Cornell Medicine, New York, NY, USA.
3
Buck Institute for Research on Aging, Novato, CA, USA. mbrand@buckinstitute.org.

Abstract

Superoxide and hydrogen peroxide produced by mitochondria play important roles in various physiological and pathological processes. This chapter describes a plate-based method to measure rates of superoxide and/or hydrogen peroxide production at specific sites in isolated mitochondria.

KEYWORDS:

Amplex UltraRed; Hydrogen peroxide; Mitochondria; Superoxide

PMID:
29851006
DOI:
10.1007/978-1-4939-7831-1_16
[Indexed for MEDLINE]

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