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Biosci Biotechnol Biochem. 2018 Sep;82(9):1606-1614. doi: 10.1080/09168451.2018.1475211. Epub 2018 May 30.

Analysis of polyamine biosynthetic- and transport ability of human indigenous Bifidobacterium.

Author information

1
a Faculty of Bioresources and Environmental Sciences , Ishikawa Prefectural University , Nonoichi , Ishikawa , Japan.
2
b Graduate School of Medical and Dental Sciences , Niigata University , Niigata , Japan.

Erratum in

  • Erratum. [Biosci Biotechnol Biochem. 2018]

Abstract

Bifidobacteria are members of the human intestinal microbiota, being numerically dominant in the colon of infants, and also being prevalent in the large intestine of adults. In this study, we measured the concentrations of major polyamines (putrescine, spermidine, and spermine) in cells and culture supernatant of 13 species of human indigenous Bifidobacterium at growing and stationary phase. Except for Bifidobacterium bifidum and Bifidobacterium gallicum, 11 species contained spermidine and/or spermine when grown in Gifu-anaerobic medium (GAM). However, Bifidobacterium scardovii and Bifidobacterium longum subsp. infantis, which contain spermidine when grown in GAM, did not contain spermidine when grown in polyamine-free 199 medium. Of the tested 13 Bifidobacterium species, 10 species showed polyamine transport ability. Combining polyamine concentration analysis in culture supernatant and in cells, with basic local alignment search tool analysis suggested that novel polyamine transporters are present in human indigenous Bifidobacterium.

ABBREVIATIONS:

Put: putrescine; Spd: spermidine; Spm: spermine; GAM: Gifu anaerobic medium; BHI: brain-heart infusion.

KEYWORDS:

Bifidobacterium; Gut bacteria; Polyamine biosynthesis; Polyamine transport

PMID:
29847302
DOI:
10.1080/09168451.2018.1475211
[Indexed for MEDLINE]

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