Format

Send to

Choose Destination
Exp Ther Med. 2018 Jun;15(6):4961-4971. doi: 10.3892/etm.2018.6058. Epub 2018 Apr 13.

Transfer of multiple loci of donor's genes to induce recipient tolerance in organ transplantation.

Author information

1
Thoracic Surgery Department, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, P.R. China.
2
Thoracic Oncology Laboratory, Department of Surgery, Helen Diller Family Comprehensive Cancer Center, University of California San Francisco, San Francisco, CA 94143-1724, USA.
3
Medical Experiment and Test Center, Capital Medical University, Beijing 100054, P.R. China.
4
Department of Molecular Biology, Capital Medical University, Beijing 100054, P.R. China.
5
Experimental Center, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, P.R. China.

Abstract

Donor organ rejection remains a significant problem. The present study aimed to assess whether transferring a donor's major histocompatibility complex (MHC) genes to the recipient could mitigate rejection in organ transplantation. Seven loci of MHC genes from donor mice were amplified and ligated into vectors; the vectors either contained one K locus, seven loci or were empty (control). The vectors were subsequently injected into the thymus of recipients (in heterotransplants, recipient rats received the vector containing one K locus), following which donor mouse hearts were transplanted. Following the transplantation of allograft and heterograft, electrocardiosignals were viable for a significantly longer duration in recipient mice and rats receiving the donor histocompatibility-2 complex (H-2)d genes compared with those in controls, and in mice that received seven vectors compared with those receiving one vector. Mixed lymphocyte cultures containing cells from these recipients proliferated significantly less compared with mixed lymphocyte cultures containing controls. Also, hearts from H-2d genes-treated recipients demonstrated less lymphocyte infiltration and necrosis compared with the control recipient. The present study concluded that allograft and heterograft rejection may be mitigated by introducing the donor's MHC into the recipient; transferring seven loci has been demonstrated to be more effective than transferring one locus.

KEYWORDS:

major histocompatibility complex; thymus; transplantation

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center