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Proc Natl Acad Sci U S A. 2018 Jun 19;115(25):6482-6487. doi: 10.1073/pnas.1803861115. Epub 2018 May 29.

Genome-wide regulation of light-controlled seedling morphogenesis by three families of transcription factors.

Author information

1
State Key Laboratory of Protein and Plant Gene Research, School of Advanced Agricultural Sciences and School of Life Sciences, Peking University, 100871 Beijing, China.
2
College of Life Sciences, Capital Normal University, 100048 Beijing, China.
3
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520.
4
State Key Laboratory of Protein and Plant Gene Research, School of Advanced Agricultural Sciences and School of Life Sciences, Peking University, 100871 Beijing, China; deng@pku.edu.cn shangwei.zhong@pku.edu.cn.

Abstract

Three families of transcription factors have been reported to play key roles in light control of Arabidopsis seedling morphogenesis. Among them, bHLH protein PIFs and plant-specific protein EIN3/EIN3-LIKE 1 (EIN3/EIL1) accumulate in the dark to maintain skotomorphogenesis. On the other hand, HY5 and HY5 HOMOLOG (HYH), two related bZIP proteins, are stabilized in light and promote photomorphogenic development. To systemically investigate the transcriptional regulation of light-controlled seedling morphogenesis, we generated HY5ox/pifQein3eil1, which contained mutations of EIN3/EIL1 and four PIF genes (pifQein3eil1) and overexpression of HY5 Our results show that dark-grown HY5ox/pifQein3eil1 seedlings display a photomorphogenesis highly similar to that of wild-type seedlings grown in continuous light, with remarkably enhanced photomorphogenic phenotypes compared with the pifQ mutants. Consistent with the genetic evidence, transcriptome analysis indicated that PIFs, EIN3/EIL1, and HY5 are dominant transcription factors in collectively mediating a wide range of light-caused genome-wide transcriptional changes. Moreover, PIFs and EIN3/EIL1 independently control the expression of light-regulated genes such as HLS1 to cooperatively regulate apical hook formation, hypocotyl elongation, and cotyledon opening and expansion. This study illustrates a comprehensive regulatory network of transcription activities that correspond to specific morphological aspects in seedling skotomorphogenesis and photomorphogenesis.

KEYWORDS:

EIN3/EIL1; PIFs; ethylene signal; light signal; plant photobiology

PMID:
29844157
PMCID:
PMC6016779
DOI:
10.1073/pnas.1803861115
[Indexed for MEDLINE]
Free PMC Article

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