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Exp Cell Res. 2018 Aug 15;369(2):179-186. doi: 10.1016/j.yexcr.2018.05.030. Epub 2018 May 26.

The development of methods for primary mast cells in vitro and ex vivo: An historical review.

Author information

1
Department of General Surgery, Shanghai Tenth People's Hospital of Tongji University, Tongji University School of Medicine, China; Department of General Surgery, Shanghai Clinical Medical College, Anhui Medical University, China.
2
Department of General Surgery, Shanghai Tenth People's Hospital of Tongji University, Tongji University School of Medicine, China.
3
Cancer Center, Houston Methodist Research Institute, United States. Electronic address: zwei@houstonmethodist.org.
4
Department of General Surgery, Shanghai Tenth People's Hospital of Tongji University, Tongji University School of Medicine, China; Department of General Surgery, Shanghai Clinical Medical College, Anhui Medical University, China. Electronic address: leejiyu@sina.com.

Abstract

Mast cells (MCs) are tissue-based stationary effector cells that form the immune system's first-line defense against various challenges. They are developed from the bone marrow-derived progenitors to complete their differentiation and maturation in the tissues where they eventually establish residence. MCs have been implicated in many diseases, such as allergy, parasitic infection, and neoplastic disorders. Immortalized MC lines, such as RBL-2H3, HMC-1, and LAD-2, are useful for investigating the biological functions of MC only to some extents due to the restriction of degranulation evaluation, in vivo injection and other factors. Over the past few decades, technologies for acquiring primarily MCs have been continually optimized, and novel protocols have been proposed. However, no relevant publications have analyzed and summarized these techniques. In this review, the classical approaches for extracting MCs are generalized, and new methods with potential values are introduced. We also evaluate the advantages and applicability of diverse MC models. Since MCs exhibit substantial plasticity and functional diversity due to different origins, it is both necessary and urgent to select a reliable and suitable source of MCs for a particular study.

KEYWORDS:

Bone marrow; Cord blood; IL-3; In vitro culture; Mast cell; SCF; iPS

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