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Carbohydr Polym. 2018 Sep 1;195:515-524. doi: 10.1016/j.carbpol.2018.04.126. Epub 2018 May 1.

Investigation of composition, structure and bioactivity of extracellular polymeric substances from original and stress-induced strains of Thraustochytrium striatum.

Author information

1
Department of Environmental Engineering and Earth Sciences, Clemson University, 342 Computer Court, Anderson, SC 29625, USA.
2
Department of Biological Sciences, Life Sciences Facility, Clemson University, Clemson, SC 29634, USA.
3
Joint Institute of Biological Science, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA.
4
Department of Biological Sciences, 132 Long Hall, Clemson University, Clemson, SC 29634, USA.
5
Joint Institute of Biological Science, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA; Department of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN 37996, USA; Department of Forestry, Wildlife, and Fisheries, Center for Renewable Carbon, University of Tennessee Institute of Agriculture, Knoxville, TN 37996, USA.
6
Department of Chemical and Biomolecular Engineering, Clemson University, Clemson, SC 29634-0909, USA.
7
Department of Environmental Engineering and Earth Sciences, Clemson University, 342 Computer Court, Anderson, SC 29625, USA. Electronic address: zheng9@clemson.edu.

Abstract

This paper was the first to study extracellular polymeric substances (EPSs) of Thraustochytrium striatum on composition, structure and bioactivities. Two strains of T. striatum including original (ori) and high-biomass (mut) strains (induced by high-nitrogen stress) were compared. The EPSs from both strains mainly contained polysaccharide (41-64%, w/w, dry basis) and protein (25-40%, w/w, dry basis), which was shown by the morphology study with an AFM. The monosaccharide profile of the EPS polysaccharide was consisted of glucose, galactose, arabinose, and trace amount of xylose. Glucose and arabinose took up to 82-90% (w/w, dry basis) of the total polysaccharide. The structure and functional groups of EPSs were determined by FTIR and NMR. The NMR results revealed that the major structural linkages of the polysaccharides of both ori and mut EPSs were 1 → 6-β-glucan and 1 → 4-α-galactan branched with l-α-arabinose. The EPSs were found to have anti-tumor activities against mouse melanoma B16-F0, human prostate carcinoma DU145, human cervical carcinoma HeLa, and human lung carcinoma A549. The EPSs also showed antioxidant and anti-inflammatory activities and antibacterial activity against Pseudomonas aeruginosa.

KEYWORDS:

Bioactivity; Exopolysaccharide; Extracellular polymeric substances; Marine protist; Nitrogen stress; Thraustochytrium striatum

PMID:
29805006
DOI:
10.1016/j.carbpol.2018.04.126
[Indexed for MEDLINE]

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