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Angew Chem Int Ed Engl. 2018 Sep 17;57(38):12370-12374. doi: 10.1002/anie.201802843. Epub 2018 Jun 14.

Proteomic Analysis of Single Mammalian Cells Enabled by Microfluidic Nanodroplet Sample Preparation and Ultrasensitive NanoLC-MS.

Author information

1
Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, 99354, USA.
2
Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, 99354, USA.
3
Department of Pediatrics-Neonatology, University of Rochester Medical Center, Rochester, NY, 14642, USA.

Abstract

We report on the quantitative proteomic analysis of single mammalian cells. Fluorescence-activated cell sorting was employed to deposit cells into a newly developed nanodroplet sample processing chip, after which samples were analyzed by ultrasensitive nanoLC-MS. An average of circa 670 protein groups were confidently identified from single HeLa cells, which is a far greater level of proteome coverage for single cells than has been previously reported. We demonstrate that the single-cell proteomics platform can be used to differentiate cell types from enzyme-dissociated human lung primary cells and identify specific protein markers for epithelial and mesenchymal cells.

KEYWORDS:

cell typing; droplets; microfluidics; single-cell proteomics; ultrasensitive LC-MS

PMID:
29797682
PMCID:
PMC6261339
[Available on 2019-09-17]
DOI:
10.1002/anie.201802843
[Indexed for MEDLINE]

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