Format

Send to

Choose Destination
J Mater Sci Mater Med. 2018 May 23;29(6):73. doi: 10.1007/s10856-018-6082-0.

In vitro cell response on CP-Ti surfaces functionalized with TGF-β1 inhibitory peptides.

Author information

1
Escola Universitària Salesiana de Sarrià, Pg. Sant Joan Bosco 74, 08017,, Barcelona, Spain. psevilla@euss.es.
2
Biomaterials, Biomechanics and Tissue Engineering group, Technical University of Catalonia, Pav. E, Av. Diagonal 647,, Barcelona, Spain. psevilla@euss.es.
3
School of Dentistry, University of Granada, Campus Universitario de Cartuja, s/n, 18071,, Granada, Spain.
4
Independent Researcher, Madrid, Spain.
5
Biomaterials, Biomechanics and Tissue Engineering group, Technical University of Catalonia, Pav. E, Av. Diagonal 647,, Barcelona, Spain.
6
School of Dentistry, Universitat Internacional de Catalunya, C/ Inmaculada 22,, Barcelona, Spain.
7
Department of Restorative Sciences, MDRCBB-Minnesota Dental Research Center for Biomechanics and Biomaterials, University of Minnesota School of Dentistry, 16-250A Moos Tower, 515 Delaware St. SE, Minneapolis, Minneapolis, MN, 55455,, USA.

Abstract

Osseointegration of implants is conversely related to the generation of a fibrous tissue capsule around the implant by the host environment. Although TGF-β1 plays many roles in regeneration processes, it is the cytokine to be mostly associated to the production of fibrotic tissue and thus, its inhibition has demonstrated to be beneficial to prevent several fibrotic reactions. Surface biofunctionalization enables the immobilization of biologically active molecules on an implant surface to tailor the biological response of the host. Here, we studied in vitro biological effects of biofunctionalized CP-Ti surfaces with a TGF-β1 inhibitor peptide, P144. A reliable biofunctionalization process that tethers P144 peptides to commercially pure titanium was developed. Differentiation of human mesenchymal stem cells, osteoblasts and fibroblasts on P144-functionalized and control surfaces was assessed at the gene expression and protein production levels. Results showed that P144-functionalized surfaces reduced expression and production of fibrotic differentiation markers and increased osteoblastic differentiation markers. Therefore, biofunctionalization of surfaces with TGF-β1 inhibitor peptides are an alternative promising strategy for inducing osseointegration around medical devices and implants.

PMID:
29796827
DOI:
10.1007/s10856-018-6082-0
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center