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Diabetes. 2018 Aug;67(8):1512-1523. doi: 10.2337/db17-1488. Epub 2018 May 23.

The Transplantation of ω3 PUFA-Altered Gut Microbiota of fat-1 Mice to Wild-Type Littermates Prevents Obesity and Associated Metabolic Disorders.

Author information

1
University of Bourgogne Franche-Comté, L'Unité de Formation Sciences de la Vie, de la Terre et de l'Environnement, Lipides Nutrition Cancer UMR1231, Dijon, France.
2
INSERM, Lipides Nutrition Cancer UMR1231, Dijon, France.
3
LipSTIC LabEx, Fondation de Coopération Scientifique Bourgogne-Franche Comté, Dijon, France.
4
Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1347, Agroécologie, Dijon, France.
5
Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1062 Centre de Biologie pour la Gestion des Populations (Institut National de la Recherche Agronomique, L'Institut de Recherche pour le Développement, Centre de coopération Internationale en Recherche Agronomique pour le Développement, Montpellier SupAgro), Montferrier-sur-Lez, France.
6
CellImap-Cellular Imaging Platform, Faculté de Médecine et Pharmacie, Dijon, France.
7
Clermont Université, Université d'Auvergne, Unité de Nutrition Humaine, Clermont-Ferrand, France.
8
Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1019, Unité de Nutrition Humaine, Centre de Recherche en Nutrition Humaine Auvergne, Clermont-Ferrand, France.
9
Institut National de la Recherche Agronomique , Unité Mixte de Recherche 1397, CarMeN Laboratory, Lyon 1 University, INSERM U1060, Institut National des Sciences Appliquées of Lyon, Rockefeller and Charles Merieux Lyon-Sud Medical Universities, Lyon, France.
10
Université Catholique de Louvain, Welbio (Walloon Excellence in Life Sciences and BIOtechnology), Louvain Drug Research Institute, Metabolism and Nutrition Research Group, Brussels, Belgium.
11
L'Unité de Formation Médecine, Université de Bourgogne, Dijon, France.
12
University of Bourgogne Franche-Comté, L'Unité de Formation Sciences de la Vie, de la Terre et de l'Environnement, Lipides Nutrition Cancer UMR1231, Dijon, France michel.narce@u-bourgogne.fr jerome.bellenger@u-bourgogne.fr.

Abstract

Altering the gut microbiome may be beneficial to the host and recently arose as a promising strategy to manage obesity. Here, we investigated the relative contribution of ω3 polyunsaturated fatty acid (PUFA)-mediated alterations in the microbiota to metabolic parameter changes in mice. Four groups were compared: male fat-1 transgenic mice (with constitutive production of ω3 PUFAs) and male wild-type (WT) littermates fed an obesogenic (high fat/high sucrose [HFHS]) or a control diet. Unlike WT mice, HFHS-fed fat-1 mice were protected against obesity, glucose intolerance, and hepatic steatosis. Unlike WT mice, fat-1 mice maintained a normal barrier function, resulting in a significantly lower metabolic endotoxemia. The fat-1 mice displayed greater phylogenic diversity in the cecum, and fecal microbiota transplantation from fat-1 to WT mice was able to reverse weight gain and to normalize glucose tolerance and intestinal permeability. We concluded that the ω3 PUFA-mediated alteration of gut microbiota contributed to the prevention of metabolic syndrome in fat-1 mice. It occurred independently of changes in the PUFA content of host tissues and may represent a promising strategy to prevent metabolic disease and preserve a lean phenotype.

PMID:
29793999
DOI:
10.2337/db17-1488
[Indexed for MEDLINE]
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