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Biodegradation. 2018 Aug;29(4):323-337. doi: 10.1007/s10532-018-9835-z. Epub 2018 May 22.

Exploring potential applications of a novel extracellular polymeric substance synthesizing bacterium (Bacillus licheniformis) isolated from gut contents of earthworm (Metaphire posthuma) in environmental remediation.

Author information

1
Enviromicrobiology, Ecotoxicology and Ecotechnology Research Laboratory, Department of Ecological Studies, University of Kalyani, Nadia, Kalyani, 741235, West Bengal, India. biswajoy2008@gmail.com.
2
International Centre for Ecological Engineering, University of Kalyani, Kalyani, 741235, West Bengal, India. biswajoy2008@gmail.com.
3
Enviromicrobiology, Ecotoxicology and Ecotechnology Research Laboratory, Department of Ecological Studies, University of Kalyani, Nadia, Kalyani, 741235, West Bengal, India.
4
Department of Biotechnology, SGB Amravati University, Amravati, 444602, Maharashtra, India.
5
School of Architecture and Civil Engineering, Institute of Foundation Engineering, Water- and Waste-Management, Soil- and Groundwater-Management, University of Wuppertal, Pauluskirchstraße 7, 42285, Wuppertal, Germany.
6
Department of Environment and Energy, University of Sejong, 98 Gunja-Dong, Guangjin-Gu, Seoul, South Korea.
7
Department of Soil and Water Sciences, Faculty of Agriculture, University of Kafrelsheikh, Kafr El-Sheikh, 33 516, Egypt.
8
Department of Marine Science, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700 019, West Bengal, India.
9
Formerly Vice Chancellor's Office, Sambalpur University, JyotiVihar, Sambalpur, Odisha, 768019, India.
10
Department of Zoology, University of Kalyani, Nadia, Kalyani, 741235, West Bengal, India.
11
Department of Soil Protection, State Office for Environmental Protection of Saxony-Anhalt, ReideburgerStraße 47, 06116, Halle/Saale, Germany.
12
Office of the Dean, Faculty of Applied Sciences, University of Sri Jayewardenepura, Nugegoda, Sri Lanka.
13
Institute of Soil and Environmental Sciences, University of Agriculture Faisalabad, Faisalabad, 38040, Pakistan.
14
MARUM and Department of Geosciences, University of Bremen, 28359, Bremen, Germany.

Abstract

The aim was to isolate, characterize, and explore potentials of gut bacteria from the earthworm (Metaphire posthuma) and imply these bacteria for remediation of Cu(II) and Zn(II). An extracellular polymeric substance (EPS) producing gut bacteria (Bacillus licheniformis strain KX657843) was isolated and identified based on 16S rRNA sequencing and phylogenetic analysis. The strain showed maximum tolerance of 8 and 6 mM for Cu(II) and Zn(II) respectively. It removed 34.5% of Cu(II) and 54.4% of Zn(II) at 25 mg L-1 after 72 and 96 h incubation respectively. The bacteria possessed a great potential to produce indole acetic acid (38.49 μg mL-1) at 5 mg mL-1 L-tryptophan following 12 days incubation. The sterilized seeds of mung beans (Vigna radiata) displayed greater germination and growth under bacterium enriched condition. We observed that the bacterial strain phosphate solubilization ability with a maximum of 204.2 mg L-1 in absence of Cu(II) and Zn(II). Endowed with biosurfactant property the bacterium exhibited 24% emulsification index. The bacterium offered significant potential of plant growth promotion, Cu(II) and Zn(II) removal, and as such this study is the first report on EPS producing B. licheniformis KX657843 from earthworm which can be applied as powerful tool in remediation programs of Cu(II) and Zn(II) contaminated sites.

KEYWORDS:

Biosurfactant; Gut bacteria; Heavy metal removal; Indole acetic acid; Phosphate solubilization; Vigna radiata

PMID:
29789975
DOI:
10.1007/s10532-018-9835-z
[Indexed for MEDLINE]

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