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Ann Hematol. 2018 Sep;97(9):1563-1575. doi: 10.1007/s00277-018-3332-7. Epub 2018 May 22.

Distinctive CD8+ T cell and MHC class I signatures in polycythemia vera patients.

Author information

1
CICS-UBI-Health Sciences Research Center, University of Beira Interior, Av. Infante D. Henrique, 6200-506, Covilhã, Portugal.
2
FCS-Faculty of Health Sciences, University of Beira Interior, Covilhã, Portugal.
3
IPG-Instituto Politécnico da Guarda, Guarda, Portugal.
4
CHTV-Centro Hospitalar Tondela-Viseu, Viseu, Portugal.
5
ULSG-Unidade Local de Saúde, Guarda, Portugal.
6
CHCB-Centro Hospitalar Cova da Beira, Covilhã, Portugal.
7
CICS-UBI-Health Sciences Research Center, University of Beira Interior, Av. Infante D. Henrique, 6200-506, Covilhã, Portugal. arosa@fcsaude.ubi.pt.
8
FCS-Faculty of Health Sciences, University of Beira Interior, Covilhã, Portugal. arosa@fcsaude.ubi.pt.

Abstract

Polycythemia vera (PV) is a myeloproliferative neoplasm characterized by overproduction of red blood cells. We have performed a comprehensive characterization of blood immune cells for expression of naïve and memory receptors as well as β2m-associated and β2m-free MHC class I heavy chains, also known as closed and open conformers, respectively, in PV patients and age-matched controls (CTR). We show that the peripheral CD3+CD8+ T cell pool in PV patients is clearly divided into two discrete populations, a more granular CD3+CD8high T cell population enriched in effector-memory CD45RA+ T cells (CD8+ TEMRA) when compared to CTR (P < 0.001), and a less granular CD3+CD8int T cell population that is completely absent in the CTR group (78 vs. 0%, P < 0.001) and is a mixture of naïve (CD8+ TN) and CD8+ TEMRA cells expressing intermediate levels of CD28, i.e., CD3+CD8intCD28int. While the percentage of CD3+CD8int TN cells correlated positively with the number of erythrocytes, the percentage of CD3+CD8int TEMRA correlated negatively with the number of platelets. Finally, we report that PV patients' lymphocytes and monocytes display lower levels of closed (W6/32+) MHC-I conformers at the cell surface while exhibiting increased amounts of open (HC-10+) MHC-I conformers. The implications of this distinctive immune signature are discussed.

KEYWORDS:

CD3+CD8intCD28int; Effector-memory lymphocytes; JAK2; Myeloproliferative neoplasm; Open HLA class I conformers

PMID:
29789880
DOI:
10.1007/s00277-018-3332-7
[Indexed for MEDLINE]

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