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Development. 2018 May 22;145(10). pii: dev162354. doi: 10.1242/dev.162354.

CLERK is a novel receptor kinase required for sensing of root-active CLE peptides in Arabidopsis.

Author information

1
Department of Plant Molecular Biology, University of Lausanne, Biophore Building, CH-1015 Lausanne, Switzerland.
2
Structural Plant Biology Laboratory, Department of Botany and Plant Biology, University of Geneva, CH-1211 Geneva, Switzerland.
3
Department of Plant Molecular Biology, University of Lausanne, Biophore Building, CH-1015 Lausanne, Switzerland christian.hardtke@unil.ch.

Abstract

CLAVATA3/EMBRYO SURROUNDING REGION (CLE) peptides are secreted endogenous plant ligands that are sensed by receptor kinases (RKs) to convey environmental and developmental inputs. Typically, this involves an RK with narrow ligand specificity that signals together with a more promiscuous co-receptor. For most CLEs, biologically relevant (co-)receptors are unknown. The dimer of the receptor-like protein CLAVATA 2 (CLV2) and the pseudokinase CORYNE (CRN) conditions perception of so-called root-active CLE peptides, the exogenous application of which suppresses root growth by preventing protophloem formation in the meristem. clv2 as well as crn null mutants are resistant to root-active CLE peptides, possibly because CLV2-CRN promotes expression of their cognate receptors. Here, we have identified the CLE-RESISTANT RECEPTOR KINASE (CLERK) gene, which is required for full sensing of root-active CLE peptides in early developing protophloem. CLERK protein can be replaced by its close homologs, SENESCENCE-ASSOCIATED RECEPTOR-LIKE KINASE (SARK) and NSP-INTERACTING KINASE 1 (NIK1). Yet neither CLERK nor NIK1 ectodomains interact biochemically with described CLE receptor ectodomains. Consistently, CLERK also acts genetically independently of CLV2-CRN We, thus, have discovered a novel hub for redundant CLE sensing in the root.

KEYWORDS:

Arabidopsis; BAM3; CLE peptide; Protophloem; Receptor kinase; Root

PMID:
29789310
PMCID:
PMC6001375
DOI:
10.1242/dev.162354
[Indexed for MEDLINE]
Free PMC Article

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