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PLoS One. 2018 May 22;13(5):e0197829. doi: 10.1371/journal.pone.0197829. eCollection 2018.

Analysis of microRNAs in familial Mediterranean fever.

Author information

1
Department of Pediatric Rheumatology, Schneider Children's Medical Center of Israel, Petach Tikva, Israel.
2
Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
3
Department of Internal Medicine F, Chaim Sheba Medical Center, Tel Hashomer, Israel.

Abstract

OBJECTIVES:

Although Familial Mediterranean fever (FMF) is categorized as autosomal recessive, frequent exceptions to this model exist and therefore we aimed to search epigenetic modifications in this disease.

METHODS:

Ten M694V homozygous FMF patients (the most severe phenotype) were recruited for this study. Patients with inflammatory flare were excluded. Total RNA was extracted from peripheral blood, and microRNA expression profiled using NanoString nCounter technology. These patients were compared to 10 healthy age- and sex-matched controls.

RESULTS:

Seven hundred nighty-eight mature human miRNAs were probed, 103 of which had expression levels above the negative control probes. Seven miRNAs showed significant differences in expression in samples from FMF patients compared to healthy controls: four miRNAs were upregulated (miR-144-3p, miR-21-5p, miR-4454, and miR-451a), and three were downregulated (miR-107, let-7d-5p, and miR-148b-3p).

CONCLUSION:

In this pilot study, we identified epigenetic modifications in clinically quiescent FMF patients. More studies are required for exploration of their contribution to FMF pathogenesis and their potential role as clinical biomarkers.

PMID:
29787577
PMCID:
PMC5963758
DOI:
10.1371/journal.pone.0197829
[Indexed for MEDLINE]
Free PMC Article

Conflict of interest statement

The study was funded by a commercial source: Novartis Inc. The corresponding author hereby declares on behalf of all authors that there are no financial, non-financial professional or personal competing interests between this funder to any of the authors in relationship to this work and manuscript. (Competing interest is defined as anything that interferes with, or could reasonably be perceived as interfering with, the full and objective presentation, peer review, editorial decision making, or publication of research or non-research articles submitted to one of the journals). This does not alter our adherence to PLOS ONE policies on sharing data and materials (as detailed online in the guide for authors http://journals.plos.org/plosone/s/competing-interests).

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