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Murine GLUTag Cells.


The Impact of Food Bioactives on Health: in vitro and ex vivo models [Internet]. Cham (CH): Springer; 2015. Chapter 21.

Author information

TNO, Zeist, The Netherlands
Cork, Ireland
Instituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM), Madrid, Spain
Norwegian University of Life Sciences, As, Norway
Institute of Food Research Norwich Research Park, Norwich, Norfolk, United Kingdom
Universidad Autonoma de Madrid, Madrid, Spain
Polish Academy of Sciences, Olsztyn, Poland
Wageningen University, Wageningen, The Netherlands
Department of Physiology, University of Toronto, Toronto, ON, Canada
Department of Medicine, University of Toronto, Toronto, ON, Canada


The incretin hormone, glucagon-like peptide-1 (GLP-1) serves as a link between ingested nutrients and insulin secretion. Because of the anti-diabetic properties exerted by GLP-1, several derivative drugs are currently in the market for the treatment of patients with type 2 diabetes. Over the past few years, several cell lines have been established as useful models for the study of the GLP-1-secreting enteroendocrine L-cells. This review focuses on the murine GLUTag cell line, derived from colonic tumours of transgenic mice expressing large T antigen under the control of the proglucagon promoter. These cells are widely used to examine the effects of food components on GLP-1 secretion, as well as the signaling pathways underlying nutrient-induced GLP-1 release. The effects of different food components, as well as of nutrient-related signals, on GLP-1 secretion, and the protocols for the maintenance and use of the murine GLUTag L-cell line will be discussed.

Copyright 2015, The Author(s).

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