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Murine GLUTag Cells.

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The Impact of Food Bioactives on Health: in vitro and ex vivo models [Internet]. Cham (CH): Springer; 2015. Chapter 21.

Author information

1
TNO, Zeist, The Netherlands
2
Cork, Ireland
3
Instituto de Investigación en Ciencias de la Alimentación (CIAL, CSIC-UAM), Madrid, Spain
4
Norwegian University of Life Sciences, As, Norway
5
Institute of Food Research Norwich Research Park, Norwich, Norfolk, United Kingdom
6
Universidad Autonoma de Madrid, Madrid, Spain
7
Polish Academy of Sciences, Olsztyn, Poland
8
Wageningen University, Wageningen, The Netherlands
9
Department of Physiology, University of Toronto, Toronto, ON, Canada
10
Department of Medicine, University of Toronto, Toronto, ON, Canada

Excerpt

The incretin hormone, glucagon-like peptide-1 (GLP-1) serves as a link between ingested nutrients and insulin secretion. Because of the anti-diabetic properties exerted by GLP-1, several derivative drugs are currently in the market for the treatment of patients with type 2 diabetes. Over the past few years, several cell lines have been established as useful models for the study of the GLP-1-secreting enteroendocrine L-cells. This review focuses on the murine GLUTag cell line, derived from colonic tumours of transgenic mice expressing large T antigen under the control of the proglucagon promoter. These cells are widely used to examine the effects of food components on GLP-1 secretion, as well as the signaling pathways underlying nutrient-induced GLP-1 release. The effects of different food components, as well as of nutrient-related signals, on GLP-1 secretion, and the protocols for the maintenance and use of the murine GLUTag L-cell line will be discussed.

Copyright 2015, The Author(s).

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