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Brain Behav. 2018 Jun;8(6):e01001. doi: 10.1002/brb3.1001. Epub 2018 May 22.

Hippocampal lipidome and transcriptome profile alterations triggered by acute exposure of mice to GSM 1800 MHz mobile phone radiation: An exploratory study.

Author information

1
Department of Cell Biology and Biophysics, Faculty of Biology, University of Athens, Zografou, Athens, Greece.
2
Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden.
3
Institute of Molecular Biology, Genetics and Biotechnology, Biomedical Research Foundation, Academy of Athens, Athens, Greece.
4
Consiglio Nazionale delle Ricerche, ISOF, Bologna, Italy.
5
Laboratory Animal Facilities, Center of Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation, Academy of Athens, Athens, Greece.
6
Department of Biomedical Engineering, University of West Attica, Athens, Greece.
7
Institute of Nanoscience and Nanotechnology (INN), NCSR Demokritos, Athens, Greece.
8
DNA Damage Laboratory, Department of Physics, School of Applied Mathematical and Physical Sciences, National Technical University of Athens (NTUA), Athens, Greece.

Abstract

BACKGROUND:

The widespread use of wireless devices during the last decades is raising concerns about adverse health effects of the radiofrequency electromagnetic radiation (RF-EMR) emitted from these devices. Recent research is focusing on unraveling the underlying mechanisms of RF-EMR and potential cellular targets. The "omics" high-throughput approaches are powerful tools to investigate the global effects of RF-EMR on cellular physiology.

METHODS:

In this work, C57BL/6 adult male mice were whole-body exposed (nExp  = 8) for 2 hr to GSM 1800 MHz mobile phone radiation at an average electric field intensity range of 4.3-17.5 V/m or sham-exposed (nSE  = 8), and the RF-EMR effects on the hippocampal lipidome and transcriptome profiles were assessed 6 hr later.

RESULTS:

The data analysis of the phospholipid fatty acid residues revealed that the levels of four fatty acids [16:0, 16:1 (6c + 7c), 18:1 9c, eicosapentaenoic acid omega-3 (EPA, 20:5 ω3)] and the two fatty acid sums of saturated and monounsaturated fatty acids (SFA and MUFA) were significantly altered (p < 0.05) in the exposed group. The observed changes indicate a membrane remodeling response of the tissue phospholipids after nonionizing radiation exposure, reducing SFA and EPA, while increasing MUFA residues. The microarray data analysis demonstrated that the expression of 178 genes changed significantly (p < 0.05) between the two groups, revealing an impact on genes involved in critical biological processes, such as cell cycle, DNA replication and repair, cell death, cell signaling, nervous system development and function, immune system response, lipid metabolism, and carcinogenesis.

CONCLUSIONS:

This study provides preliminary evidence that mobile phone radiation induces hippocampal lipidome and transcriptome changes that may explain the brain proteome changes and memory deficits previously shown by our group.

KEYWORDS:

brain; fatty acids; gene expression; membrane remodeling; radiofrequencies

PMID:
29786969
PMCID:
PMC5991598
DOI:
10.1002/brb3.1001
[Indexed for MEDLINE]
Free PMC Article

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