Format

Send to

Choose Destination
Sci Rep. 2018 May 21;8(1):7957. doi: 10.1038/s41598-018-25866-y.

A direct tissue-grafting approach to increasing endogenous brown fat.

Author information

1
Department of Biomedical Engineering, Columbia University, New York, NY, 10027, USA.
2
Department of Medicine, Division of Endocrinology and Metabolism, Medical University of Vienna, A-1090, Vienna, Austria.
3
Department of Chemical Engineering, Ankara University, 06100, Tandogan, Ankara, Turkey.
4
Department of Medicine, Division of Preventative Medicine and Nutrition, Columbia University Medical Center, New York, NY, 10032, USA.
5
Departments of Medicine and Neuroscience, Albert Einstein College of Medicine, Bronx, NY, 10461, USA.
6
Department of Surgery, Division of Plastic and Reconstructive Surgery, Columbia University Medical Center, New York, NY, 10032, USA.
7
Department of Medicine, Division of Endocrinology, Columbia University Medical Center, New York, NY, 10032, USA.
8
Department of Biomedical Engineering, Columbia University, New York, NY, 10027, USA. bg@ardentcell.com.
9
Department of Surgery, Division of Wound Healing and Regenerative Medicine, NYU Winthrop Hospital, 259 1st Street, Mineola, NY, 11501, USA. bg@ardentcell.com.
10
Ardent Cell Technologies, Inc., 423 West 127th Street, New York, NY, 10027, USA. bg@ardentcell.com.
11
Department of Biomedical Engineering, Columbia University, New York, NY, 10027, USA. ss2735@columbia.edu.

Abstract

There is widespread evidence that increasing functional mass of brown adipose tissue (BAT) via browning of white adipose tissue (WAT) could potentially counter obesity and diabetes. However, most current approaches focus on administration of pharmacological compounds which expose patients to highly undesirable side effects. Here, we describe a simple and direct tissue-grafting approach to increase BAT mass through ex vivo browning of subcutaneous WAT, followed by re-implantation into the host; this cell-therapy approach could potentially act synergistically with existing pharmacological approaches. With this process, entitled "exBAT", we identified conditions, in both mouse and human tissue, that convert whole fragments of WAT to BAT via a single step and without unwanted off-target pharmacological effects. We show that ex vivo, exBAT exhibited UCP1 immunostaining, lipid droplet formation, and mitochondrial metabolic activity consistent with native BAT. In mice, exBAT exhibited a highly durable phenotype for at least 8 weeks. Overall, these results enable a simple and scalable tissue-grafting strategy, rather than pharmacological approaches, for increasing endogenous BAT and studying its effect on host weight and metabolism.

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center