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Sci Rep. 2018 May 21;8(1):7905. doi: 10.1038/s41598-018-26349-w.

Polycomb Repressive Complex 2 attenuates the very high expression of the Arabidopsis gene NRT2.1.

Author information

1
BPMP, CNRS, INRA, SupAgro, Univ. Montpellier, Montpellier, France.
2
Institut de Biologie de l'Ecole Normale Supérieure, CNRS UMR8197, INSERM U1024, ENS, 46 rue d'Ulm, 75005, Paris, France.
3
Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRA, F-69342, Lyon, France.
4
BPMP, CNRS, INRA, SupAgro, Univ. Montpellier, Montpellier, France. antoine.martin@supagro.fr.

Abstract

PRC2 is a major regulator of gene expression in eukaryotes. It catalyzes the repressive chromatin mark H3K27me3, which leads to very low expression of target genes. NRT2.1, which encodes a key root nitrate transporter in Arabidopsis, is targeted by H3K27me3, but the function of PRC2 on NRT2.1 remains unclear. Here, we demonstrate that PRC2 directly targets and down-regulates NRT2.1, but in a context of very high transcription, in nutritional conditions where this gene is one of the most highly expressed genes in the transcriptome. Indeed, the mutation of CLF, which encodes a PRC2 subunit, leads to a loss of H3K27me3 at NRT2.1 and results, exclusively under permissive conditions for NRT2.1, in a further increase in NRT2.1 expression, and specifically in tissues where NRT2.1 is normally expressed. Therefore, our data indicates that PRC2 tempers the hyperactivity of NRT2.1 in a context of very strong transcription. This reveals an original function of PRC2 in the control of the expression of a highly expressed gene in Arabidopsis.

PMID:
29784958
PMCID:
PMC5962593
DOI:
10.1038/s41598-018-26349-w
[Indexed for MEDLINE]
Free PMC Article

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