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Sci Rep. 2018 May 21;8(1):7913. doi: 10.1038/s41598-018-25577-4.

An Oil-Free Picodrop Bioassay Platform for Synthetic Biology.

Author information

1
Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California, USA.
2
Amyris, Inc. Emeryville, California, USA.
3
Delv Bio, Sacramento, California, USA.
4
Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California, USA.
5
Chan Zuckerberg Biohub, San Francisco, California, USA.
6
Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, California, USA. arabate@gmail.com.
7
Chan Zuckerberg Biohub, San Francisco, California, USA. arabate@gmail.com.

Abstract

Droplet microfluidics enables massively-parallel analysis of single cells, biomolecules, and chemicals, making it valuable for high-throughput screens. However, many hydrophobic analytes are soluble in carrier oils, preventing their quantitative analysis with the method. We apply Printed Droplet Microfluidics to construct defined reactions with chemicals and cells incubated under air on an open array. The method interfaces with most bioanalytical tools and retains hydrophobic compounds in compartmentalized reactors, allowing their quantitation.

PMID:
29784937
PMCID:
PMC5962535
DOI:
10.1038/s41598-018-25577-4
[Indexed for MEDLINE]
Free PMC Article

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