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Talanta. 2018 Aug 15;186:521-526. doi: 10.1016/j.talanta.2018.04.011. Epub 2018 Apr 11.

Highly sensitive detection of clenbuterol in urine sample by using surface plasmon resonance immunosensor.

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Graduate School of Environmental Sciences, Hokkaido University, Sapporo 060-0810, Japan; Department of Chemistry, Faculty of Science, University of Rajshahi, Rajshahi 6205, Bangladesh.
New Business Development Office, Corporate R&D Division Ushio INC., 1-6-5 Marunouchi, Chiyoda-ku, Tokyo 100-8150, Japan. Electronic address:
Yabegawa electronics. Co., Hachie-machi 65 Omuta-shi, Fukuoka 836-0847, Japan.
Graduate School of Global Food Resources, Hokkaido University, Sapporo 060-8589, Japan; Global Station for Food, Land and Water Resources, Global Institute for Collaborative Research and Education, Hokkaido University, Sapporo 060-8589, Japan.


This study proposed the filtration method for removal of inhibitors from real urine samples for immunoassay without centrifuge. Although the inhibitors could not be removed by the physical filtration, the carboxyl group terminated silica effectively removed the inhibitors. In a low pH, antibody formed aggregation due to the protonation. We propose to adjust pH of the sample solution by adding a phosphate buffer solution (pH 7.5). As a result of pretreatment, the SPR immunosensing achieved the SPR signal of 45 mdeg and a low limit of detection with 100 ppq (100 fg mL-1).


Clenbuterol; Indirect competitive inhibition; Inhibitor determination; SPR immunosensing; Urine

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