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Nutrients. 2018 May 19;10(5). pii: E643. doi: 10.3390/nu10050643.

Lactobacillus plantarum Strain Ln4 Attenuates Diet-Induced Obesity, Insulin Resistance, and Changes in Hepatic mRNA Levels Associated with Glucose and Lipid Metabolism.

Author information

1
Research group of Traditional Food, Korea Food Research Institute, Wanju 55365, Korea. ejlee@kfri.re.kr.
2
Research group of Traditional Food, Korea Food Research Institute, Wanju 55365, Korea. jarasasa2@gmail.com.
3
Research Group of Natural Materials and Metabolism, Korea Food Research Institute, Wanju 55365, Korea. sylee09@kfri.re.kr.
4
Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Korea. lnk11@konkuk.ac.kr.
5
Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Korea. hdpaik@konkuk.ac.kr.
6
Research Group of Healthcare, Korea Food Research Institute, Wanju 55365, Korea. silim@kfri.re.kr.

Abstract

The prevalence of obesity and associated metabolic disorders, including diabetes and cardiovascular disease, is rapidly becoming a severe global health problem. Recent reports have suggested that the alteration of the gut ecosystem through the consumption of probiotics and fermented foods, such as yogurt and Kimchi, can significantly impact obesity and Type 2 diabetes (T2D)-related biomarkers. In this study, we screened over 400 strains of lactic acid bacteria (LAB) that were isolated from fermented foods to identify potent anti-obesogenic and diabetic probiotics in vitro. Of the strains tested, Lactobacillus plantarum Ln4 (Ln4), which was obtained from napa cabbage kimchi, significantly reduced lipid accumulation and stimulated glucose uptake in 3T3-L1 adipocytes. Oral administration of Ln4 reduced weight gain and epididymal fat mass in mice fed on a high-fat diet (HFD). Total plasma triglyceride level was significantly lower in mice that were treated Ln4 as compared with mice fed HFD. The protein levels of adipokines such as C-reactive protein (CRP), insulin-like growth factor binding proteins-3 (IGFBP-3), and monocyte chemoattractant protein-1 (MCP-1) decreased in white adipose tissues of Ln4-treated mice. Furthermore, these mice exhibited a significant reduction of insulin resistance index (HOMA-IR) and the improvement of glucose tolerance (OGTT) and insulin response (ITT) following Ln4 administration. This was associated with changes in several hepatic gene expressions (increased mRNA levels of IRS2, Akt2, AMPK, LPL, and reduced CD36) that regulate glucose and lipid metabolism. Taken together, these results indicate that in vitro and in vivo Ln4 treatment attenuates diet-induced obesity and T2D biomarkers, highlighting the potential of Ln4 as a therapeutic probiotic agent for metabolic disorders.

KEYWORDS:

high fat diet; insulin resistance; insulin signaling; lactobacillus plantarum Ln4; metabolic disorder; obesity

PMID:
29783731
PMCID:
PMC5986522
DOI:
10.3390/nu10050643
[Indexed for MEDLINE]
Free PMC Article

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