Format

Send to

Choose Destination
J Ethnopharmacol. 2018 Sep 15;223:113-121. doi: 10.1016/j.jep.2018.05.020. Epub 2018 May 18.

Tumor inhibitory effects of intravesical Ganoderma lucidum instillation in the syngeneic orthotopic MB49/C57 bladder cancer mice model.

Author information

1
School of Nursing, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China. Electronic address: john.yuen@polyu.edu.hk.
2
School of Nursing, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China. Electronic address: deejay.sy.mak@polyu.edu.hk.
3
SH Ho Urology Centre, Department of Surgery, The Chinese University of Hong Kong, Sha Tin, N.T., Hong Kong, China. Electronic address: eddie@surgery.cuhk.edu.hk.
4
School of Medical and Health Science, Tung Wah College, Homantin, Kowloon, Hong Kong, China. Electronic address: dannygohel@twc.edu.hk.
5
SH Ho Urology Centre, Department of Surgery, The Chinese University of Hong Kong, Sha Tin, N.T., Hong Kong, China. Electronic address: ngcf@surgery.cuhk.edu.hk.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Ganoderma lucidum (GL) has been traditionally used in oriental medicine as superior health tonic, and there are numerous scientific evidences of its antitumorigenic activities.

AIM OF THE STUDY:

To evaluate the intravesical chemopreventive effects of ethanol extract of GL (GLe) on bladder cancer.

MATERIALS AND METHODS:

Intravesical therapy is defined as the direct instillation of a liquid drug into bladder through a catheter. Bacille Calmette-Guerin(BCG) solution is applied intravesically as a conventional immunotherapy for preventing recurrence of bladder cancer. By adopting the MB49/C57 bladder cancer mice model, an overall 60 MB49-implanted mice were randomized into 3 groups and treated according to 3 treatment arms, including GLe, BCG and PBS. Additionally, wild-type mice without MB49 cell inoculation and treated with PBS were used as the negative control group. Testing agents were instilled intravesically for 2 h and repeated after one week for evaluating the effects on preventing the tumor formation and growth. The treated-mice were closely monitored for major adverse effects.

RESULTS:

GLe demonstrated more potent cytotoxic effects than BCG on MB49 cells, although both in dose-dependent manner. In the MB49-implanted mice, 80 µg/ml GLe was shown to delay the tumor formation by one week, whereas the averaged tumor volume measured at endpoint was 3.6-fold and 4.6-fold smaller than that of the BCG or PBS, respectively. However, no significant effects were observed on body weight and hematuria.

CONCLUSION:

Current findings in mice suggested intravesical GLe therapy as an effective and safe chemopreventive strategy for inhibiting bladder tumor formation.

KEYWORDS:

Bladder cancer; Ganoderma lucidum; Intravesical therapy; MB49; Urothelial carcinoma

PMID:
29783018
DOI:
10.1016/j.jep.2018.05.020
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center