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Biochim Biophys Acta Proteins Proteom. 2018 Aug;1866(8):849-856. doi: 10.1016/j.bbapap.2018.05.009. Epub 2018 May 16.

Revealing A-Raf functions through its interactome.

Author information

1
Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, 727 Jingming South Road, Kunming 650500, China; Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 Jingming South Road, Kunming 650500, China.
2
Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 Jingming South Road, Kunming 650500, China; The First People's Hospital of Yunnan Province, 157 Jinbi Road, Kunming 650032, China.
3
Institute of Biomedical Sciences, Minhang hospital, Fudan University, 170 Xinsong Road, Shanghai 201199, China.
4
State Key Laboratory of Genetic Engineering, Department of Biochemistry, School of Life Sciences, Fudan University, 2005 Songhu Road, Shanghai 200433, China.
5
Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 Jingming South Road, Kunming 650500, China.
6
Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 Jingming South Road, Kunming 650500, China. Electronic address: aslxj@mail.ustc.edu.cn.
7
Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 Jingming South Road, Kunming 650500, China. Electronic address: tianruixu@kmust.edu.cn.

Abstract

A-Raf is a member of the Raf kinase family. Unlike B-Raf and C-Raf, the functions of A-Raf remain obscure. To gain more insight into the biological functions of A-Raf, we investigated the A-Raf interactome using proteomics. We found 132 proteins that interact with A-Raf and confirmed the interaction of 12 of these proteins with A-Raf by western blotting. Our data suggested that A-Raf regulates apoptosis, RNA catabolism, GTPase activity, and cell adhesion by interacting with proteins located in different cellular compartments. We identified all ten hallmarks of cancer in these interacting proteins, suggesting that A-Raf is involved in carcinogenesis. Our results also indicated that A-Raf may play a role in different diseases and signaling pathways. These findings have identified potential regulators of A-Raf and provide a systemic insight into its biological functions.

KEYWORDS:

A-Raf; Cancer; Cell signaling; Interacting protein

PMID:
29777862
DOI:
10.1016/j.bbapap.2018.05.009
[Indexed for MEDLINE]

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