Format

Send to

Choose Destination
Biomed Pharmacother. 2018 Aug;104:275-279. doi: 10.1016/j.biopha.2018.05.018. Epub 2018 May 25.

Ozone therapy prevents the onset of dysplasia in HPV16-transgenic mice-A pre-clinical efficacy and safety analysis.

Author information

1
Department of Veterinary Sciences, UTAD, Vila Real, Portugal. Electronic address: cecilia_peirone@yahoo.com.ar.
2
Department of Veterinary Sciences, UTAD, Vila Real, Portugal; Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal. Electronic address: vero.mestre11@gmail.com.
3
Department of Veterinary Sciences, UTAD, Vila Real, Portugal; Center for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal.
4
Department of Veterinary Sciences, UTAD, Vila Real, Portugal; Zootechnics Department, UTAD, Vila Real, Portugal.
5
Department of Veterinary Sciences, UTAD, Vila Real, Portugal; Laboratory for Process Engineering Environment Biotechnology and Energy (LEPABE) Chemical Engineering Department, Faculty of Engineering, University of Porto (FEUP), Porto, Portugal; Molecular Oncology and Viral Pathology Group, IPO-Porto Research Center (CI-IPOP), Portuguese Institute of Oncology of Porto (IPO-Porto), Porto, Portugal. Electronic address: rmcosta@fe.up.pt.
6
Advanced Polytechnic and University Cooperative (CESPU), Institute of Research and Advanced Training in Health Sciences and Technologies (IINFACTS), Gandra, Portugal; Research Center in Physical Activity, Health and Leisure (CIAFEL), Faculty of Sports, University of Porto, Porto, Portugal.
7
Molecular Oncology and Viral Pathology Group, IPO-Porto Research Center (CI-IPOP), Portuguese Institute of Oncology of Porto (IPO-Porto), Porto, Portugal; Faculty of Medicine, University of Porto (FMUP), Porto, Portugal; CEBIMED, Faculty of Health Sciences, Fernando Pessoa University, Porto, Portugal; LPCC Research Department, Portuguese League against Cancer (NRNorte), Porto, Portugal.
8
Laboratory for Process Engineering Environment Biotechnology and Energy (LEPABE) Chemical Engineering Department, Faculty of Engineering, University of Porto (FEUP), Porto, Portugal.
9
Critical Care Department, University Hospital of Braga, Braga, Portugal.

Abstract

Infection with high-risk human papillomavirus (HPV), most often HPV16, is associated with the development of anogenital and oropharyngeal cancers. Recently, ozone therapy was reported to have considerable efficacy against rabbit VX2 tumors, induced by the cottontail rabbit papillomavirus. The present study aims to determine whether similar results can be obtained in HPV16-transgenic mice, possibly paving the way for new therapeutic options against HPV-induced cancers. HPV16-transgenic and wild-type, female, 20 weeks-old mice were injected intraperitoneally with medical O3/O2 (80░mL/kg, at O3 50░μg/mL), once a day, for 5 consecutive days. The animals were sacrificed at 25 weeks-old, and skin samples were analyzed histologically to study tumour progression. Blood and internal organ samples were used to study toxicological parameters. 85.7% of untreated transgenic mice showed dysplastic skin lesions, compared with 28.6% of O3-treated mice. This was associated with a marked reduction of dermal inflammation associated with those lesions. No significant changes were observed in any toxicological parameters. These preliminary results support the hypothesis that O3 therapy is effective against papillomavirus-induced lesions, particularly against those induced by the most common high-risk virus, HPV16. Further studies are needed to confirm the mechanisms underlying these effects.

KEYWORDS:

Cancer; HPV16; Human papillomavirus; Mouse; Ozone; Pre-clinical

PMID:
29775895
DOI:
10.1016/j.biopha.2018.05.018
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center