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Microbes Infect. 2019 Jan - Feb;21(1):56-62. doi: 10.1016/j.micinf.2018.04.005. Epub 2018 Oct 10.

Identification of novel HLA-A11-restricted T-cell epitopes in the Ebola virus nucleoprotein.

Author information

1
Laboratory Animal Center of the Academy of Military Medical Science, Beijing, 100071, China; State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China.
2
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China.
3
INSERM U1197 (ex U1014), University of Paris-Sud, Hospital Paul Brousse, Villejuif, France.
4
Laboratory Animal Center of the Academy of Military Medical Science, Beijing, 100071, China.
5
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China. Electronic address: yszhou@bmi.ac.cn.
6
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, 100071, China. Electronic address: sunsh01@163.com.
7
Laboratory Animal Center of the Academy of Military Medical Science, Beijing, 100071, China. Electronic address: zenglin1965@126.com.

Abstract

The Ebola virus (EBOV) is a very contagious virus that is highly fatal in humans and animals. The largest epidemic was in West Africa in 2014, in which over 11,000 people died. However, to date, there are no licensed vaccines against it. Studies show that CD4+ and CD8+ T-cell responses, especially cytotoxic T-lymphocyte (CTL) responses, play key roles in protecting individuals from EBOV infection. Since HLA-restricted epitope vaccines are likely to be effective and safe immunization strategies for infectious diseases, the present study screened for CTL epitopes in the EBOV-nucleoprotein that are restricted by HLA-A11 (a common allele in Chinese people). Predictive computer analysis of the amino-acid sequence of EBOV-nucleoprotein identified ten putative HLA-A11-restricted epitopes. ELISPOT assay of immunized HLA-A11/DR1 transgenic mice showed that five (GR-9, VR-9, EK-9, PK-9, and RK-9) induced effective CTL responses. Additional epitope analyses will aid the design of epitope vaccines against EBOV.

KEYWORDS:

Ebola virus; HLA-A11-restricted epitope; Vaccine

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