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Plant J. 2018 May 18. doi: 10.1111/tpj.13973. [Epub ahead of print]

LFR is functionally associated with AS2 to mediate leaf development in Arabidopsis.

Lin X1,2,3,4, Gu D1,4, Zhao H1,2,3,4, Peng Y4, Zhang G1,2,3,4, Yuan T1,4, Li M4, Wang Z1,4, Wang X1,4, Cui S1,2,3,4.

Author information

1
Hebei Key Laboratory of Molecular and Cellular Biology, Hebei Normal University, Hebei, 050024, China.
2
Key Laboratory of Molecular and Cellular Biology of Ministry of Education, Hebei Normal University, Hebei, 050024, China.
3
Hebei Collaboration Innovation Center for Cell Signaling, Hebei Normal University, Hebei, 050024, China.
4
College of Life Science, Hebei Normal University, Hebei, 050024, China.

Abstract

Leaves are essential organs for plants. We previously identified a functional gene possibly encoding a component of the SWI/SNF complex named Leaf and Flower Related (LFR) in Arabidopsis thaliana. Loss-of-function mutants of LFR displayed obvious defects in leaf morphogenesis, indicating its vital role in leaf development. Here an allelic null mutant of ASYMMETRIC LEAVES2 (AS2), as2-6, was isolated as an enhancer of lfr-1 in petiole length, vasculature pattern and leaf margin development. The lfr as2 double-mutants showed enhanced ectopic expression of BREVIPEDICELLUS (BP) compared with each of the single-mutants, which is consistent with their synergistic genetic enhancement in multiple BP-dependent development processes. Moreover, LFR and several putative subunits of the SWI/SNF complex interacted physically with AS2. LFR associated with BP chromatin in an AS1-AS2-dependent manner to promote the nucleosome occupancy for appropriate BP repression in leaves. Taken together, our findings reveal that LFR and the SWI/SNF complex play roles in leaf development at least partly by repressing BP transcription as interacting factors of AS2, which expounds our understanding of BP repression at the chromatin structure level in leaf development.

KEYWORDS:

Arabidopsis thaliana ; ASYMMETRIC LEAVES2 (AS2); BREVIPEDICELLUS (BP); Leaf and Flower Related (LFR); leaf

PMID:
29775508
DOI:
10.1111/tpj.13973
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