Format

Send to

Choose Destination
Int J Radiat Biol. 2018 Jul;94(7):664-670. doi: 10.1080/09553002.2018.1478161. Epub 2018 Jun 19.

Evaluation of chromosomal aberrations induced by 188Re-dendrimer nanosystem on B16f1 melanoma cells.

Author information

1
a Area de Radiofarmacia, Facultad de Ciencias , Centro de Investigaciones Nucleares, Universidad de la República , Montevideo , Uruguay.
2
b Laboratorio de Señalización Celular y Nanobiología , Instituto de Investigaciones Biológicas Clemente Estable, Ministerio de Educación y Cultura , Montevideo , Uruguay.
3
d Departamento de Química Orgánica, Facultad de Química , Universidad de la República , Montevideo , Uruguay.
4
c Laboratorio de Epigenética e Inestabilidad Genómica , Instituto de Investigaciones Biológicas Clemente Estable, Ministerio de Educación y Cultura , Montevideo , Uruguay.

Abstract

PURPOSE:

To study the rhenium-188 labeling of polyamidoamine (PAMAM) generation 4 (G4) dendrimer and its evaluation on biodistribution and chromosomal aberrations in melanoma cells induced by ionizing radiation as potential treatment agent.

MATERIALS AND METHODS:

Dendrimers were first conjugated with Suc-HYNIC (succinimidyl 6-hydrazinopyridine-3-carboxylic acid hydrochloride). Dendrimer-HYNIC was then incubated with 188ReO4-. Biodistribution was performed administrating 188Re-dendrimer to normal (NM) or melanoma-bearing mice (MBM). Chromosome aberration test was conducted in order to measure treatment capacity of 188Re-dendrimer in melanoma cells.

RESULTS:

Radiolabeling yield of dendrimer was approx. 70%. Biodistribution studies in NM showed blood clearance with hepatic and renal depuration. MBM showed a similar pattern of biodistribution with tumor uptake of 6% of injected dose. Aberrant metaphases quantified in control cells were 7%, increasing to 29.5% in cells treated with 15μCi (0.555 MBq) of 188Re-dendrimer for 24 h.

CONCLUSIONS:

188Re-dendrimer can produce double-stranded breaks in DNA induced by ionizing radiation in melanoma cells in vitro.

KEYWORDS:

188Re; Dendrimer; anti-tumor therapy; chromosomal aberrations; melanoma

Supplemental Content

Full text links

Icon for Taylor & Francis
Loading ...
Support Center