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J Trace Elem Med Biol. 2018 Dec;50:629-633. doi: 10.1016/j.jtemb.2018.05.004.

The effect of the deuterium depleted water on the biological activity of the eukaryotic cells.

Author information

1
Peoples Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya St., Moscow, 117198, Russian Federation.
2
Peoples Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya St., Moscow, 117198, Russian Federation; Shemyakin-Ovchinnikov Institute of bioorganic chemistry, Moscow, Russian Federation.
3
State Institute of Genetic and Regenerative Medicine of National Medical Academy of Sciences of Ukraine, Kyiv 04114, Ukraine; Biotechnology Laboratory ilaya.regeneration, Medical Company ilaya®, Kyiv 03115, Ukraine.
4
Peoples Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya St., Moscow, 117198, Russian Federation; Dumanskii Institute of Colloid and Water Chemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine. Electronic address: zlatskiy@ukr.net.
5
Dumanskii Institute of Colloid and Water Chemistry, National Academy of Sciences of Ukraine, Kiev, Ukraine.
6
Peoples Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya St., Moscow, 117198, Russian Federation; Federal Research Center of Epidemiology and Microbiology named Gamalei, Moscow, Russian Federation.

Abstract

Here we show the dependence of the unicellular biosensor S.ambigua lifespan on the water D/H isotopic composition. This dependence is bell-shaped with descents both in case of deficiency or excess of deuterium in water. The influence of the water D/H isotopic composition on the cell culture proliferative potential and colony forming efficiency in vitro was tested on the human dermal fibroblasts. We observed that the deuterium depleted water stimulates cell colony formation at the early passages. The dynamics of the cell doubling index in the deuterium depleted water-based growth medium showed higher proliferation potential compared to the water with normal isotopic composition. Using scratch assay, we have also studied the impact of the growth medium D/H isotopic composition on the cell motility of human cancer cell lines A549 and HT29. We have shown that the deuterium depleted water considerably suppressed cancer cell lines amoeboid movement in vitro.

KEYWORDS:

Colony-Forming ability; Deuterium depleted water; Human dermal fibroblasts; In vitro; Isotopic composition of water; Monolayer scratch method; Proliferative potential; Spirotox

PMID:
29773469
DOI:
10.1016/j.jtemb.2018.05.004
[Indexed for MEDLINE]

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