Hilic MS/MS determination of amino acids in herbs of Fumaria schleicheri L., Ocimum basilicum L., and leaves of Corylus avellana L

Nat Prod Res. 2019 Jul;33(13):1961-1963. doi: 10.1080/14786419.2018.1477145. Epub 2018 May 18.

Abstract

The aim of research was to study the content of amino acids using in extracts of Fumaria schleicheri L., Ocimum basilicum L., and Corylus avellana L. by HILIC MS/MS method. Separation of amino acids in the samples was carried out with Acquity H-class UPLC system (Waters, Milford, USA) equipped with SeQuant ZIC-Hilic collumn (2.1 × 150 mm, 3.5 μm) (Merck Millipore, Darmstadt, Germany). The MS/MS fragment ion chromatograms of the test solutions established the presence of 19 amino acids. The obtained results have shown that O. basilicum L. characterized the highest concentrations of different neurogenic amino acids (128.1 mg/kg), comparing with F. schleicheri L. and C. avellana L. (57.72 and 52.91 mg/kg, respectively).

Keywords: L.; Amino acids; HILIC MS/MS.

MeSH terms

  • Amino Acids / analysis*
  • Corylus / chemistry*
  • Fumaria / chemistry*
  • Hydrophobic and Hydrophilic Interactions
  • Ocimum basilicum / chemistry*
  • Plant Extracts / analysis
  • Plant Leaves / chemistry
  • Tandem Mass Spectrometry / methods*

Substances

  • Amino Acids
  • Plant Extracts