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Nutrients. 2018 May 17;10(5). pii: E635. doi: 10.3390/nu10050635.

Leucine Supplementation Does Not Attenuate Skeletal Muscle Loss during Leg Immobilization in Healthy, Young Men.

Author information

1
Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, P.O. Box 61 6200 MD Maastricht, The Netherlands. lisette.degroot@wur.nl.
2
Department of Human Nutrition, Wageningen University, P.O. Box 17 6700 AA Wageningen, The Netherlands. lisette.degroot@wur.nl.
3
Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, P.O. Box 61 6200 MD Maastricht, The Netherlands. astrid_horstman@hotmail.com.
4
Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, P.O. Box 61 6200 MD Maastricht, The Netherlands. g.marzucanassr@maastrichtuniversity.nl.
5
Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, P.O. Box 61 6200 MD Maastricht, The Netherlands. j.vankranenburg@maastrichtuniversity.nl.
6
Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, P.O. Box 61 6200 MD Maastricht, The Netherlands. joey.smeets@maastrichtuniversity.nl.
7
Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, P.O. Box 61 6200 MD Maastricht, The Netherlands. cas.fuchs@maastrichtuniversity.nl.
8
Department of Surgery, Maastricht University Medical Centre+, P.O. Box 616 6200 MD Maastricht, The Netherlands. anniek.janssen@mumc.nl.
9
Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, P.O. Box 61 6200 MD Maastricht, The Netherlands. tim.snijders@maastrichtuniversity.nl.
10
Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, P.O. Box 61 6200 MD Maastricht, The Netherlands. Lex.verdijk@maastrichtuniversity.nl.
11
Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, P.O. Box 61 6200 MD Maastricht, The Netherlands. L.vanLoon@maastrichtuniversity.nl.

Abstract

BACKGROUND:

Short successive periods of physical inactivity occur throughout life and contribute considerably to the age-related loss of skeletal muscle mass. The maintenance of muscle mass during brief periods of disuse is required to prevent functional decline and maintain metabolic health.

OBJECTIVE:

To assess whether daily leucine supplementation during a short period of disuse can attenuate subsequent muscle loss in vivo in humans.

METHODS:

Thirty healthy (22 ± 1 y) young males were exposed to a 7-day unilateral knee immobilization intervention by means of a full leg cast with (LEU, n = 15) or without (CON, n = 15) daily leucine supplementation (2.5 g leucine, three times daily). Prior to and directly after immobilization, quadriceps muscle cross-sectional area (computed tomography (CT) scan) and leg strength (one-repetition maximum (1-RM)) were assessed. Furthermore, muscle biopsies were taken in both groups before and after immobilization to assess changes in type I and type II muscle fiber CSA.

RESULTS:

Quadriceps muscle cross-sectional area (CSA) declined in the CON and LEU groups (p < 0.01), with no differences between the two groups (from 7712 ± 324 to 7287 ± 305 mm² and from 7643 ± 317 to 7164 ± 328 mm²; p = 0.61, respectively). Leg muscle strength decreased from 56 ± 4 to 53 ± 4 kg in the CON group and from 63 ± 3 to 55 ± 2 kg in the LEU group (main effect of time p < 0.01), with no differences between the groups (p = 0.052). Type I and II muscle fiber size did not change significantly over time, in both groups (p > 0.05).

CONCLUSIONS:

Free leucine supplementation with each of the three main meals (7.5 g/d) does not attenuate the decline of muscle mass and strength during a 7-day limb immobilization intervention.

KEYWORDS:

amino acid; countermeasures; limb immobilization; strength

PMID:
29772844
PMCID:
PMC5986514
DOI:
10.3390/nu10050635
[Indexed for MEDLINE]
Free PMC Article

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