Objective: To in silico clone the NAD1 gene of three common parasites and analyze their bioinformatics, so as to lay the foundation for further research on the NAD gene.
Methods: By using the in silico cloning method, the full length cDNA (s) of NAD 1 genes of Clonorchis sinensis, Ascaris lumbricoides and Schistosoma japonicum were got, then their physical and chemical properties, compositions of amino acids, subcellular localizations, binary and ternary structures were contrastively analyzed.
Results: The three kinds of NAD1 proteins were similar in the relative molecular weight, subcellular localization, and physical and chemical properties. The NAD1 proteins were highly similar in binary and ternary structures of A. lumbricoides and S. japonicum. The phylogenetic analysis showed that C. sinensis, A. lumbricoides and S. japonicum belonged to the different evolutionary branches with a certain of genetic distance.
Conclusions: The three NAD1 genes got from C. sinensis, A. lumbricoides and S. japonicum by in silico cloning belong to the same gene of different species, which can be widely used in the researches of heritable variation of parasites.
[摘要] 目的 利用电子克隆法对人体寄生虫NAD1基因进行电子克隆和生物信息学分析, 为NAD1基因的进⼀步深入 研究奠定基础。方法 分别选取华支睾吸虫、蛔虫和日本血吸虫的⼀个表达序列标签 (EST) 片段作为种子序列, 以NCBI 中的EST数据库作为目标参考序列, 用电子克隆的方法分别克隆3种寄生虫的NAD1基因, 并对其编码蛋白的理化性质、 氨基酸组成、亚细胞定位、⼆级和三级结构进行对比分析。结果 3种人体寄生虫NAD1蛋白理化性质、亚细胞定位、相 对分子量、等电点差异较小, 蛔虫和日本血吸虫的NAD1蛋白在三级结构上高度相似。进化分析显示, 3种人体寄生虫的 NAD1蛋白分属不同分支, 存在⼀定的遗传距离。结论 通过电子克隆得到的3个NAD1基因是属于不同种属的同⼀基 因, 可广泛应用于研究寄生虫的种间和种内遗传变异。.
Keywords: Bioinformatics analysis; In silico cloning; NAD1 gene; Parasite.