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Biomed Res Int. 2018 Mar 25;2018:4702481. doi: 10.1155/2018/4702481. eCollection 2018.

Bauhinia variegata candida Fraction Induces Tumor Cell Death by Activation of Caspase-3, RIP, and TNF-R1 and Inhibits Cell Migration and Invasion In Vitro.

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Laboratory of Experimental Pathology, Federal University of São João del-Rei (UFSJ), West Center Campus Dona Lindu, 35.501-296 Divinópolis, MG, Brazil.
Molecular Oncology Research Center (CPOM), Barretos Cancer Hospital, 14.784-400 Barretos, SP, Brazil.
Petroleomic and Forensic Chemistry Laboratory, Department of Chemistry, Federal University of Espírito Santo (UFES), 29075-910 Vitória, ES, Brazil.
Analytical Chemistry Laboratory, Federal University of São João del-Rei (UFSJ), West Center Campus Dona Lindu, 35.501-296 Divinópolis, MG, Brazil.
Life and Health Sciences Research Institute (ICVS), Health Sciences School, University of Minho, Braga, Portugal.
ICVS/3B's-PT Government Associate Laboratory, Guimarães, Braga, Portugal.


Metastasis remains the most common cause of death in cancer patients. Inhibition of metalloproteinases (MMPs) is an interesting approach to cancer therapy because of their role in the degradation of extracellular matrix (ECM), cell-cell, and cell-ECM interactions, modulating key events in cell migration and invasion. Herein, we show the cytotoxic and antimetastatic effects of the third fraction (FR3) from Bauhinia variegata candida (Bvc) stem on human cervical tumor cells (HeLa) and human peripheral blood mononuclear cells (PBMCs). FR3 inhibited MMP-2 and MMP-9 activity, indicated by zymogram. This fraction was cytotoxic to HeLa cells and noncytotoxic to PBMCs and decreased HeLa cell migration and invasion. FR3 is believed to stimulate extrinsic apoptosis together with necroptosis, assessed by western blotting. FR3 inhibited MMP-2 activity in the HeLa supernatant, differently from the control. The atomic mass spectrometry (ESI-MS) characterization suggested the presence of glucopyranosides, D-pinitol, fatty acids, and phenolic acid. These findings provide insight suggesting that FR3 contains components with potential tumor-selective cytotoxic action in addition to the action on the migration of tumor cells, which may be due to inhibition of MMPs.

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