Format

Send to

Choose Destination
J Virol. 2018 May 16. pii: JVI.00432-18. doi: 10.1128/JVI.00432-18. [Epub ahead of print]

Identification of plant virus receptor candidates in the stylets of their aphid vectors.

Author information

1
BGPI, Univ Montpellier, CIRAD, INRA, Montpellier SupAgro, Montpellier, France.
2
Univ Lyon, INSA-Lyon, INRA, BF2I, UMR0203, F-69621, Villeurbanne, France.
3
Instituto de Ciencias Agrarias, Consejo Superior de Investigaciones Científicas (ICA-CSIC), Madrid 28006, Spain.
4
Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY, USA.
5
BGPI, Univ Montpellier, CIRAD, INRA, Montpellier SupAgro, Montpellier, France marilyne.uzest@inra.fr.

Abstract

Plant viruses transmitted by insects cause tremendous losses in most important crops around the world. The identification of receptors of plant viruses within their insect vectors is a key challenge to understanding the mechanisms of transmission, and offers an avenue for future alternative control strategies to limit viral spread. We here report the identification of two cuticular proteins within aphid mouthparts, and we provide experimental support for the role of one of them in the transmission of a non-circulative virus. These two proteins, named Stylin-01 and Stylin-02, belong to the RR-1 cuticular protein subfamily, and are highly conserved among aphid species. Using an immunolabeling approach, they were localized in the maxillary stylets of the pea aphid Acyrthosiphon pisum and the green peach aphid Myzus persicae, in the acrostyle, an organ earlier shown to harbor receptors of a non-circulative virus. A peptide motif present at the C-terminus of both Stylin-01 and Stylin-02 is readily accessible all over the surface of the acrostyle. Competition for in vitro binding to the acrostyle was observed between an antibody targeting this peptide and the helper component protein P2 of Cauliflower mosaic virus Furthermore, silencing stylin-01 but not stylin-02 gene through RNA interference decreased the efficiency of Cauliflower mosaic virus transmission by Myzus persicae These results identify the first cuticular proteins ever reported within arthropod mouthparts and distinguish Stylin-01 as the best candidate receptor for the aphid transmission of non-circulative plant viruses.IMPORTANCE Most non-circulative plant viruses transmitted by insect vectors bind to their mouthparts. They are acquired and inoculated within seconds when insects hop from plant to plant. The receptors involved remains totally elusive due to long-standing technical bottleneck in working with insect cuticle. Here we characterize the role of the two first cuticular proteins ever identified in arthropod mouthparts. A domain of these proteins is directly accessible at the surface of the cuticle of the acrostyle, an organ at the tip of aphid stylets. The acrostyle has been shown to bind a plant virus and we consistently demonstrate that one of the identified proteins is involved in viral transmission. Our findings provide an approach to identify proteins in insect mouthparts and points at unprecedented gene candidate for plant virus receptor.

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center