Send to

Choose Destination
Mol Gen Genet. 1988 Oct;214(2):313-6.

Alleviation of type I restriction in adenine methylase (dam) mutants of Escherichia coli.

Author information

Institute of Experimental Cardiology, National Cardiology Research Center, USSR Academy of Medical Sciences, Moscow.


The host-controlled EcoK-restriction of unmodified phage lambda.O is alleviated in dam mutants of Escherichia coli by 100- to 300-fold. In addition, the EcoK modification activity is substantially decreased in dam- strains. We show that type I restriction (EcoB, EcoD and EcoK) is detectably alleviated in dam mutants. However, no relief of EcoRI restriction (Type II) occurs in dam- strains and only a slight effect of dam mutation on EcoP1 restriction (Type III) is observed. We interpret the alleviation of the type I restriction in dam- strains to be a consequence of induction of the function which interferes with type I restriction systems.

[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center