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ACS Appl Mater Interfaces. 2018 Jun 6;10(22):18450-18457. doi: 10.1021/acsami.8b01584. Epub 2018 May 25.

Development of Molecularly Imprinted Polymers To Block Quorum Sensing and Inhibit Bacterial Biofilm Formation.

Author information

1
Food, Nutrition and Health Program, Faculty of Land and Food Systems , The University of British Columbia , Vancouver , British Columbia V6T 1Z4 , Canada.
2
Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology , The University of British Columbia , Vancouver V6T 1Z4 , Canada.
3
Synthetic Biology Group, MIT Synthetic Biology Center; The Center for Microbiome Informatics and Therapeutics; Research Laboratory of Electronics, Department of Biological Engineering, and Department of Electrical Engineering and Computer Science , Massachusetts Institute of Technology , Cambridge , Massachusetts 02139 , United States.
4
Broad Institute of MIT and Harvard , Cambridge , Massachusetts 02142 , United States.

Abstract

Bacterial biofilms are responsible for most clinical infections and show increased antimicrobial resistance. In this study, molecularly imprinted polymers (MIPs) were developed to specifically capture prototypical quorum sensing autoinducers [i.e., N-(3-oxododecanoyl)-l-homoserine lactone (3-oxo-C12AHL)], interrupt quorum sensing, and subsequently inhibit biofilm formation of Pseudomonas aeruginosa, an important human nosocomial pathogen. The synthesis of MIPs was optimized by considering the amount and type of the functional monomers itaconic acid (IA) and 2-hydroxyethyl methacrylate (HEMA). IA-based MIPs showed high adsorption affinity toward 3-oxo-C12AHL with an imprinting factor of 1.68. Compared to IA-based MIPs, the adsorption capacity of HEMA-based MIPs was improved fivefold. HEMA-based MIPs significantly reduced biofilm formation (by ∼65%), whereas biofilm suppression by IA-based MIPs was neutralized because of increased bacterial attachment. The developed MIPs represent promising alternative biofilm intervention agents that can be applied to surfaces relevant to clinical settings and food processing equipment.

KEYWORDS:

Pseudomonas aeruginosa; autoinducer; biofilm; molecularly imprinted polymers; quorum sensing

PMID:
29767954
DOI:
10.1021/acsami.8b01584
[Indexed for MEDLINE]

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