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Sci Rep. 2018 May 15;8(1):7603. doi: 10.1038/s41598-018-25687-z.

Resveratrol significantly improves the fertilisation capacity of bovine sex-sorted semen by inhibiting apoptosis and lipid peroxidation.

Author information

1
Embryo Biotechnology and Reproduction Laboratory and the Centre of Domestic Animal Reproduction & Breeding, Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan Western Road, Haidian District, Beijing, 100193, P.R. China.
2
Dairy Cattle Research Center, Shandong Academy of Agricultural Sciences, No. 159 North of Industry Road, Jinan, Shandong, 250131, P.R. China.
3
Livestock and Poultry Import & Export Dept, China Animal Husbandry Group (CAHG), Building 18, Block 8, 188 West Road, South 4th Ring Road, Beijing, 100070, P.R. China.
4
Embryo Biotechnology and Reproduction Laboratory and the Centre of Domestic Animal Reproduction & Breeding, Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan Western Road, Haidian District, Beijing, 100193, P.R. China. zhuhuabin@caas.cn.
5
Embryo Biotechnology and Reproduction Laboratory and the Centre of Domestic Animal Reproduction & Breeding, Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan Western Road, Haidian District, Beijing, 100193, P.R. China. zhaoxueming@caas.cn.

Abstract

The aim of this study was to test the effects of five different concentrations (0, 10-3, 10-4, 10-5, and 10-6 M) of resveratrol (Res) supplementation in bull sperm washing and fertilisation medium on levels of reactive oxygen species (ROS), phosphatidylserine (PS) externalisation, mitochondrial membrane potential (Δψm), ATP and malondialdehyde (MDA), acrosomal integrity, blastocyst rate, and blastocyst quality after in vitro fertilisation (IVF). The results for sex-sorted sperm from three bulls showed: (1) ROS and MDA levels in 10-3 M and 10-4 M Res groups were significantly lower than those of controls (P < 0.05); (2) the percentage of viable sperm, percentage of sperm with high Δψm, and the ATP content in 10-3 M and 10-4 M Res groups were significantly higher than those of controls (P < 0.05); (3) the percentage of viable sperm with acrosomal integrity, and the blastocyst percentage and quality of the 10-4 M Res group were significantly higher than those of controls (P < 0.05). In conclusion, 10-4 M Res supplementation in washing and fertilisation medium of sex-sorted bull sperm significantly decreased ROS, PS externalisation, and MDA, and protected mitochondrial function and acrosomal integrity, thereby increasing blastocyst percentage and quality following IVF.

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