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Crit Rev Biotechnol. 2018 Dec;38(8):1261-1276. doi: 10.1080/07388551.2018.1472550. Epub 2018 May 15.

Insight into carrageenases: major review of sources, category, property, purification method, structure, and applications.

Author information

1
a College of Food Science and Light Industry , Nanjing Tech University , Nanjing , PR China.
2
b Dalian Institute of Chemical Physics , Chinese Academy of Sciences (CAS) , Dalian , PR China.
3
c Institute of Process Engineering , Chinese Academy of Sciences , Beijing , PR China.

Abstract

Carrageenan, a kind of linear sulfated polysaccharides consisting of D-galactose with alternating α-1,3 and β-1,4 linkages, has been widely applied in the food and cosmetic industries as thickening and gelling agents due to excellent properties, such as gel-forming ability and chemical stability. It can be degraded by carrageenases to produce a series of even-numbered carrageenan oligosaccharides, which exhibit various fascinating functions, such as anti-inflammation, anti-coagulation, anti-tumor, and anti-thrombosis effects. Numerous carrageenases have been isolated and identified from various sources. The enzymes are grouped into three categories, namely κ-carrageenase, ι-carrageenase, and λ-carrageenase based on their substrate specificities and primary sequences, respectively. Elucidating the paradigm of the enzyme at every aspect would definitely enhance our understanding of the marine carbon cycling and natural evolution of glycoside hydrolases (GHs). The structural features of these enzymes have been fully illustrated, which will improve our knowledge of its catalytic mechanisms. In this review, we have summarized the recent progresses of major sources, category, and the enzyme's biochemical characteristics. Additionally, structural characteristics and catalytic mechanisms have been introduced in detail. We conclude with a brief discussion of the potential of the carrageenases in possible future applications in preparing functional oligosaccharides with versatile activities. This comprehensive information should be helpful regarding the application of carrageenases.

KEYWORDS:

Carrageenan; applications; carrageenase; mechanism; oligosaccharides; structure

PMID:
29764223
DOI:
10.1080/07388551.2018.1472550
[Indexed for MEDLINE]

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