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Food Chem Toxicol. 2018 Sep;119:61-65. doi: 10.1016/j.fct.2018.05.034. Epub 2018 May 12.

Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro.

Author information

1
Department of Biology and Human Genetics, Institute of Physiology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia. Electronic address: lada.zivkovic@pharmacy.bg.ac.rs.
2
The Laboratory for Radiobiology and Molecular Genetics, Institute for Nuclear Research "Vinča", University of Belgrade, Mike Petrovića Alasa 12-14, 11000 Belgrade, Serbia.
3
Biomedical Research, R&D Institute, Galenika a.d., Pasterova 2, 11000 Belgrade, Serbia.
4
Department of Biology and Human Genetics, Institute of Physiology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia.
5
Department of Clinical Sciences, Faculty of Medicine, Polytechnic University of Marche, Via Ranieri, 65, 60131, Ancona, Italy.
6
Department of Agricultural, Food and Environmental Sciences, Polytechnic University of Marche, Via Ranieri, 65, 60131, Ancona, Italy.

Abstract

Manuka honey has been widely researched regarding its biological properties, in particular its antimicrobial and antioxidant capacities. We tested the genotoxic and genoprotective properties of Manuka honey, ranging from 25-1000 μg/mL, by performing an in vitro comet assay after exposure to human whole blood. No genotoxic effect on whole blood cells was observed within the tested concentration range (p = 0.154). Then, the antigenotoxic potency of Manuka honey against oxidative DNA damage to whole blood cells was assessed. Prior to Manuka honey treatment a modest decrease of H2O2-induced DNA damage was detected in cells, with no statistical significance (p = 0.087). Post-treatment, Manuka honey displayed a stronger potential to attenuate damaged cells at all tested concentrations, with a statistical significant difference (p < 0.001), where concentrations of 25 and 100 μg/mL were most efficient. Manuka honey exhibited a marked potential to protect DNA of whole blood cells from oxidative damage induced by hydrogen peroxide in vitro.

PMID:
29763681
DOI:
10.1016/j.fct.2018.05.034
[Indexed for MEDLINE]

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