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Nucleic Acids Res. 2018 Jul 2;46(W1):W242-W245. doi: 10.1093/nar/gky354.

CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens.

Author information

1
Muséum national d'Histoire naturelle, INSERM U1154, CNRS UMR 7196, Sorbonne Universités, 43 rue Cuvier, Paris F-75231, France.
2
Genomics Institute, University of California at Santa Cruz, 1156 High Street, MS CBSE, Santa Cruz, CA 95060, USA.

Abstract

CRISPOR.org is a web tool for genome editing experiments with the CRISPR-Cas9 system. It finds guide RNAs in an input sequence and ranks them according to different scores that evaluate potential off-targets in the genome of interest and predict on-target activity. The list of genomes is continuously expanded, with more 150 genomes added in the last two years. CRISPOR tries to provide a comprehensive solution from selection, cloning and expression of guide RNA as well as providing primers needed for testing guide activity and potential off-targets. Recent developments include batch design for genome-wide CRISPR and saturation screens, creating custom oligonucleotides for guide cloning and the design of next generation sequencing primers to test for off-target mutations. CRISPOR is available from http://crispor.org, including the full source code of the website and a stand-alone, command-line version.

PMID:
29762716
PMCID:
PMC6030908
DOI:
10.1093/nar/gky354
[Indexed for MEDLINE]
Free PMC Article

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