Format

Send to

Choose Destination
Talanta. 2018 Aug 1;185:477-482. doi: 10.1016/j.talanta.2018.04.001. Epub 2018 Apr 3.

A novel near-infrared fluorescent probe for highly selective detection of cysteine and its application in living cells.

Author information

1
College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, PR China.
2
College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, PR China; Jiangsu Collaborative Innovation Center of Biomedical Functional Materials and Jiangsu Key Laboratory of Biofunctional Materials, PR China.
3
College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, PR China; Jiangsu Collaborative Innovation Center of Biomedical Functional Materials and Jiangsu Key Laboratory of Biofunctional Materials, PR China. Electronic address: wangbingxiang@njnu.edu.cn.

Abstract

A novel red-emitting fluorescent probe (DDNA) for cysteine has been rationally designed and synthesized, which exhibited a low limit of detection to Cys (0.26 μM) as well as a favorable large stokes shift (λEmEx = 128 nm). This novel fluorophore (HDM), which features a large π-conjugation system and typical intramolecular charge transfer (ICT) process, has a long emission wavelength at 631 nm. Besides that, as a turn-on fluorescent probe, it shows high selectivity and sensitivity for Cys over other metal ions and amino acids including the similar structured homocysteine (Hcy) and glutathione (GSH). Finally, the probe DDNA was successfully applied to bioimage intracellular Cys in Hela cells with low cytotoxicity.

KEYWORDS:

Bioimaging; Cysteine; Fluorescent probe; ICT

PMID:
29759230
DOI:
10.1016/j.talanta.2018.04.001
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center