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Iran Biomed J. 2019 Jan;23(1):47-56. Epub 2018 May 13.

Differential Expression Profile of ZFX Variants Discriminates Breast Cancer Subtypes

Author information

1
Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
2
Pathology and Stem Cell Research Center, Kerman University of Medical Sciences, Kerman, Iran.

Abstract

Background:

ZFX is a transcriptional regulator in embryonic stem cells and plays an important role in pluripotency and self-renewal. ZFX is widely expressed in pluripotent stem cells and is down-regulated during differentiation of embryonic stem cells. ZFX has five different variants that encode three different protein isoforms. While several reports have determined the overexpression of ZFX in a variety of somatic cancers, the expression of ZFX-spliced variants in cancer cells is not well-understood.

Methods:

We investigated the expression of ZFX variants in a series of breast cancer tissues and cell lines using quantitative PCR.

Results:

The expression of ZFX variant 1/3 was higher in tumor tissue compared to marginal tissue. In contrast, the ZFX variant 5 was down-regulated in tumor tissues. While the ZFX variant 1/3 and ZFX variant 5 expression significantly increased in low-grade tumors, ZFX variant 4 was strongly expressed in high-grade tumors, demonstrating lymphatic invasion. In addition, our result revealed a significant association between the HER2 status and the expression of ZFX-spliced variants.

Conclusion:

Our data suggest that the expression of ZFX-spliced transcripts varies between different types of breast cancer and may contribute to their tumorigenesis process. Hence, ZFX-spliced transcripts could be considered as novel tumor markers with a probable value in diagnosis, prognosis, and therapy of breast cancer.

KEYWORDS:

Breast neoplasms; Neoplastic stem cells; RNA splicing

PMID:
29753316
PMCID:
PMC6305825
[Indexed for MEDLINE]
Free PMC Article

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