Format

Send to

Choose Destination
Stem Cell Res. 2018 May;29:250-253. doi: 10.1016/j.scr.2018.04.011. Epub 2018 Apr 23.

Blood-derived integration-free iPS cell line UKBi011-A from a diagnosed male Alzheimer's disease patient with APOE ɛ4/ɛ4 genotype.

Author information

1
Institute of Reconstructive Neurobiology, Life & Brain Center, University of Bonn Medical Faculty, Bonn 53127, Germany,; DZNE German Center for Neurodegenerative Diseases (DZNE), Bonn 53175, Germany.
2
Institute of Reconstructive Neurobiology, Life & Brain Center, University of Bonn Medical Faculty, Bonn 53127, Germany.
3
DZNE German Center for Neurodegenerative Diseases (DZNE), Bonn 53175, Germany,; Department of Neurodegenerative Diseases and Geriatric Psychiatry, University Hospital Bonn, Germany.
4
Department of Neurodegenerative Diseases and Geriatric Psychiatry, University Hospital Bonn, Germany,; Department of Psychiatry and Psychotherapy, University of Cologne, Cologne, Germany,; Department of Psychiatry and Psychotherapy, University of Bonn, Bonn, Germany.
5
Institute of Reconstructive Neurobiology, Life & Brain Center, University of Bonn Medical Faculty, Bonn 53127, Germany,. Electronic address: brustle@uni-bonn.de.

Abstract

Alzheimer's disease (AD) is most the frequent neurodegenerative disease, and the APOE ε4 allele is the most prominent risk factor for late-onset AD. Here, we present an iPSC line generated from peripheral blood cells of a male AD patient employing Sendai virus vectors encoding the transcription factors OCT4, SOX2, KLF4 and c-MYC. The characterized iPSC line expresses typical human pluripotency markers and shows differentiation into all three germ layers, complete reprogramming vector clearance, a normal SNP genotype and maintenance of the APOE ε4/ε4 allele.

PMID:
29753274
DOI:
10.1016/j.scr.2018.04.011
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center