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J Biomed Mater Res A. 2018 Nov;106(11):2795-2804. doi: 10.1002/jbm.a.36450. Epub 2018 Oct 5.

Nanoceria-loaded injectable hydrogels for potential age-related macular degeneration treatment.

Author information

1
Department of Ophthalmology, the University of North Carolina, Chapel Hill, North Carolina, 27599.
2
Carolina Institute for NanoMedicine, the University of North Carolina, Chapel Hill, North Carolina, 27599.
3
Division of Pharmacoengineering & Molecular Pharmaceutics, Eshelman School of Pharmacy, The University of North Carolina, Chapel Hill, North Carolina, 27599.

Abstract

The major purpose of this article is to evaluate oligochitosan coated cerium oxide nanoparticles (OCCNPs) alginate laden injectable hydrogels and their potential treatment for age-related macular degeneration (AMD). The water soluble OCCNPs were loaded within injectable hydrogels as antioxidative agents. The release of OCCNPs from hydrogel, radical scavenging properties, and biocompatibility were evaluated and calculated in vitro. The effects of OCCNP laden hydrogel downregulating expression of angiogenic proteins and proinflammatory cytokines were quantified in human retinal pigment epithlium-19 (ARPE-19) and umbilical endothelium cell lines. The hydrogels behaved with moderate swelling and controllable degradation. The laden OCCNPs were released in a controlled manner in vitro during two months of testing. The OCCNP loaded hydrogels exhibited robust antioxidative properties in oxygen radical absorbance capacity tests and reduced apoptosis in H2 O2 -induced ARPE-19 cells. Furthermore, OCCNP loaded injectable hydrogels are biocompatible and suppressed the ipopolysaccharides-induced inflammation response in ARPE-19 cells, and inhibited expression of vascular endothelium growth factor in human ARPE-19 and umbilical endothelium cell lines. The alginate-gelatin injectable hydrogel loaded OCCNPs are biocompatible and have high potential in protecting cells from apoptosis, angiogenesis, and production of proinflammatory cytokines in AMD cellular models.

KEYWORDS:

AMD; alginate injectable hydrogel; cellular models; cerium oxide nanoparticle

PMID:
29752862
PMCID:
PMC6231995
[Available on 2019-11-01]
DOI:
10.1002/jbm.a.36450

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