Send to

Choose Destination
Biomed Chromatogr. 2018 May 11:e4279. doi: 10.1002/bmc.4279. [Epub ahead of print]

Metabolomic comparison between wild Ophiocordyceps sinensis and artificial cultured Cordyceps militaris.

Author information

Zhengzhou Key Laboratory of Medicinal Resources Research, Institute of Nanostructured Functional Materials, Huanghe Science and Technology College, Zhengzhou, Henan, China.
Modern Research Center for Traditional Chinese Medicine of Shanxi University, Taiyuan, Shanxi, People's Republic of China.
Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, Henan, China.
China Pharmaceutical University, Nanjing, Jiangsu, China.


A systematic study on the metabolome differences between wild Ophiocordyceps sinensis and artificial cultured Cordyceps militaris was conducted using liquid chromatography-mass spectrometry. Principal component analysis and orthogonal projection on latent structure-discriminant analysis results showed that C. militaris grown on solid rice medium (R-CM) and C. militaris grown on tussah pupa (T-CM) evidently separated and individually separated from wild O. sinensis, indicating metabolome difference among wild O. sinensis, R-CM and T-CM. The metabolome differences between R-CM and T-CM indicated that C. militaris could accommodate to culture medium by differential metabolic regulation. Hierarchical clustering analysis was further performed to cluster the differential metabolites and samples based on their metabolic similarity. The higher content of amino acids (pyroglutamic acid, glutamic acid, histidine, phenylalanine and arginine), unsaturated fatty acid (linolenic acid and linoleic acid), peptides, mannitol, adenosine and succinoadenosine in O. sinensis make it as an excellent choice as a traditional Chinese medicine for invigoration or nutritional supplementation. Similar compositions with O. sinensis and easy cultivation make artificially cultured C. militaris a possible alternative to O. sinensis.


Cordyceps militaris; Ophiocordyceps sinensis; mass spectrometry; metabolomics


Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center