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Biomaterials. 2018 Sep;178:401-412. doi: 10.1016/j.biomaterials.2018.04.057. Epub 2018 May 3.

Tissue adhesive, rapid forming, and sprayable ECM hydrogel via recombinant tyrosinase crosslinking.

Author information

1
Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, 151-742, Republic of Korea; School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-742, Republic of Korea.
2
Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, 151-742, Republic of Korea; School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-742, Republic of Korea; Institute of Bioengineering, Institute of Molecular Biology and Genetics, Seoul National University, Seoul, 151-742, Republic of Korea.
3
School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-742, Republic of Korea.
4
Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, 151-742, Republic of Korea; School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-742, Republic of Korea; Institute of Chemical Processes, Seoul National University, Seoul, 151-742, Republic of Korea. Electronic address: nshwang@snu.ac.kr.
5
Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, 151-742, Republic of Korea; School of Chemical and Biological Engineering, Seoul National University, Seoul, 151-742, Republic of Korea; Institute of Bioengineering, Institute of Molecular Biology and Genetics, Seoul National University, Seoul, 151-742, Republic of Korea. Electronic address: byungkim@snu.ac.kr.

Abstract

We report on a tissue adhesive hydrogel based on novel recombinant tyrosinase mediated crosslinking. The adhesive hydrogels were fabricated by the site-directed coupling of tyramine-conjugated hyaluronic acid (HA_t, 1% w/v) and gelatin (3% w/v) (HG_gel) with novel tyrosinase derived from Streptomyces avermitilis (SA_Ty). The enzyme-based crosslinking by SA_Ty was fast, with less than 50 s for complete gelation, and the SA_Ty based crosslinking enhanced the physical properties and adhesive strength of the hydrogel significantly with the native tissue samples. Furthermore, by optimizing the injection conditions, we tailored the enzyme-based crosslinking hydrogels to be injectable and sprayable with a medical syringe and commercial airbrush nozzle, respectively. An in vivo analysis of the adhesive hydrogel showed a negligible immune reaction. In this study, demonstrate that the novel enzyme-based crosslinking hydrogel has a robust potential in tissue engineering and regenerative medicine.

KEYWORDS:

ECM hydrogel; Injectable hydrogel; Sprayable hydrogel; Tissue adhesive; Tyrosinase

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