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Cell Microbiol. 2018 Sep;20(9):e12858. doi: 10.1111/cmi.12858. Epub 2018 May 30.

Mycobacteria employ two different mechanisms to cross the blood-brain barrier.

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Medical Microbiology and Infection Control, VU Medical Center, Amsterdam, The Netherlands.
Paediatric Infectious Diseases and Immunology, VU Medical Center, Amsterdam, The Netherlands.
Cell Biology and Histology, Electron Microscopy Centre Amsterdam, Academic Medical Centre, Amsterdam, The Netherlands.
Molecular Cell Biology and Immunology, Amsterdam Neuroscience, VU Medical Center, Amsterdam, The Netherlands.


Central nervous system (CNS) infection by Mycobacterium tuberculosis is one of the most devastating complications of tuberculosis, in particular in early childhood. In order to induce CNS infection, M. tuberculosis needs to cross specialised barriers protecting the brain. How M. tuberculosis crosses the blood-brain barrier (BBB) and enters the CNS is not well understood. Here, we use transparent zebrafish larvae and the closely related pathogen Mycobacterium marinum to answer this question. We show that in the early stages of development, mycobacteria rapidly infect brain tissue, either as free mycobacteria or within circulating macrophages. After the formation of a functionally intact BBB, the infiltration of brain tissue by infected macrophages is delayed, but not blocked, suggesting that crossing the BBB via phagocytic cells is one of the mechanisms used by mycobacteria to invade the CNS. Interestingly, depletion of phagocytic cells did not prevent M. marinum from infecting the brain tissue, indicating that free mycobacteria can independently cause brain infection. Detailed analysis showed that mycobacteria are able to cause vasculitis by extracellular outgrowth in the smaller blood vessels and by infecting endothelial cells. Importantly, we could show that this second mechanism is an active process that depends on an intact ESX-1 secretion system, which extends the role of ESX-1 secretion beyond the macrophage infection cycle.


ESX-1 secretion; Trojan horse mechanism; blood-brain barrier; tuberculosis; tuberculous meningitis; zebrafish

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