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RNA. 2018 Aug;24(8):1018-1027. doi: 10.1261/rna.065516.117. Epub 2018 May 10.

Human angiogenin is a potent cytotoxin in the absence of ribonuclease inhibitor.

Author information

1
Graduate Program in Cell and Molecular Biology, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
2
Department of Biochemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
3
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
4
Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.

Abstract

Angiogenin (ANG) is a secretory ribonuclease that promotes the proliferation of endothelial cells, leading to angiogenesis. This function relies on its ribonucleolytic activity, which is low for simple RNA substrates. Upon entry into the cytosol, ANG is sequestered by the ribonuclease inhibitor protein (RNH1). We find that ANG is a potent cytotoxin for RNH1-knockout HeLa cells, belying its inefficiency as a nonspecific catalyst. The toxicity does, however, rely on the ribonucleolytic activity of ANG and a cytosolic localization, which lead to the accumulation of particular tRNA fragments (tRFs), such as tRF-5 Gly-GCC. These up-regulated tRFs are highly cytotoxic at physiological concentrations. Although ANG is well-known for its promotion of cell growth, our results reveal that ANG can also cause cell death.

KEYWORDS:

CRISPR; RNA processing; oxidative stress ribonuclease inhibitor; tRNA-derived fragment

PMID:
29748193
PMCID:
PMC6049508
[Available on 2019-08-01]
DOI:
10.1261/rna.065516.117
[Indexed for MEDLINE]

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