Nanoparticle Tracking for Protein Aggregation Research

Xiaomeng Lu; Regina M Murphy

doi:10.1007/978-1-4939-7811-3_7

Nanoparticle Tracking for Protein Aggregation Research

Methods Mol Biol. 2018:1777:145-158. doi: 10.1007/978-1-4939-7811-3_7.

Authors

Xiaomeng Lu¹, Regina M Murphy²

Affiliations

¹ Biophysics Program and Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, USA.
² Biophysics Program and Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, WI, USA. regina.murphy@wisc.edu.

Abstract

Protein aggregates are the pathological agents in several neurodegenerative disorders such as Alzheimer's and Huntington's disease. In the pharmaceutical industry, protein aggregation poses significant challenges to the manufacture of biologics. Nanoparticle tracking is an emerging technology that allows particle-by-particle measurement of aggregate size and concentration. The technique is solution based, and requires no labeling. Here we describe protocols for using nanoparticle tracking in protein aggregation research, and provide a few examples for illustrative purposes.

Keywords: Amyloid; Diffusion; Light scattering; Nanoparticles; Protein aggregation.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Nanoparticles*
Protein Aggregates*
Protein Aggregation, Pathological
Proteins / chemistry*
Research*

Substances

Protein Aggregates
Proteins

Grants and funding

R01 AG033493/AG/NIA NIH HHS/United States